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dc.contributor.authorBEVITORI, R.
dc.contributor.authorOLIVEIRA, M. B.
dc.contributor.authorGROSSI-DE-SÁ, M. F.
dc.contributor.authorLANNA, A. C.
dc.contributor.authorSILVEIRA, R. D. da
dc.contributor.authorPETROFEZA, S.
dc.date.accessioned2018-07-12T01:02:29Z-
dc.date.available2018-07-12T01:02:29Z-
dc.date.created2015-01-23
dc.date.issued2014
dc.identifier.citationGenetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1006581-
dc.descriptionDrought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleSelection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought.
dc.typeArtigo de periódico
dc.date.updated2018-07-12T01:02:29Zpt_BR
dc.subject.thesagroArroz
dc.subject.thesagroOryza sativa
dc.subject.thesagroDoença de planta
dc.subject.thesagroBrusone
dc.subject.thesagroGenética molecular
dc.subject.thesagroSeca
dc.subject.nalthesaurusGene expression
dc.subject.nalthesaurusRice
dc.subject.nalthesaurusMagnaporthe oryzae
dc.subject.nalthesaurusBlast disease
riaa.ainfo.id1006581
riaa.ainfo.lastupdate2018-07-11
dc.identifier.doihttp://dx.doi.org/10.4238/2014.November.27.7
dc.contributor.institutionROSANGELA BEVITORI, CNPAF
dc.contributor.institutionMARILIA BARROS OLIVEIRAeng
dc.contributor.institutionMARIA FATIMA GROSSI DE SA, CENARGENeng
dc.contributor.institutionANNA CRISTINA LANNA, CNPAFeng
dc.contributor.institutionRICARDO DIÓGENES DA SILVEIRAeng
dc.contributor.institutionSILVANA PETROFEZA, UFG.eng
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