Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1007369
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dc.contributor.authorZHOU, G.pt_BR
dc.contributor.authorPEREIRA, J. F.pt_BR
dc.contributor.authorDELHAIZE, E.pt_BR
dc.contributor.authorZHOU, M.pt_BR
dc.contributor.authorMAGALHAES, J. V.pt_BR
dc.contributor.authorRYAN, P. R.pt_BR
dc.date.accessioned2015-01-30T11:11:11Zpt_BR
dc.date.available2015-01-30T11:11:11Zpt_BR
dc.date.created2015-01-30pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationJournal of Experimental Botany, London, v. 65, n. 9, p. 2381-2390, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1007369pt_BR
dc.descriptionMalate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tolerance of an important crop species.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCitratopt_BR
dc.subjectTolerância ao alumíniopt_BR
dc.subjectTransgênicopt_BR
dc.titleEnhancing the aluminium tolerance of barley by expressing the citrate transporter genes SbMATE and FRD3.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-02-01T11:11:11Zpt_BR
dc.subject.thesagroCevadapt_BR
dc.subject.thesagroSolo Ácidopt_BR
riaa.ainfo.id1007369pt_BR
riaa.ainfo.lastupdate2015-02-01pt_BR
dc.identifier.doi10.1093/jxb/eru121pt_BR
dc.contributor.institutionUNIVERSITY OF TASMANIA; JORGE FERNANDO PEREIRA, CNPT; CSIRO PLANT INDUSTRY; UNIVERSITY OF TASMANIA; JURANDIR VIEIRA DE MAGALHAES, CNPMS; CSIRO PLANT INDUSTRY.pt_BR
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