Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1007801
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorCARDOSO, D. C.pt_BR
dc.contributor.authorMARTINATI, J. C.pt_BR
dc.contributor.authorGIACHETTO, P. F.pt_BR
dc.contributor.authorVIDAL, R. O.pt_BR
dc.contributor.authorCARAZZOLLE, M. F.pt_BR
dc.contributor.authorPADILHA, L.pt_BR
dc.contributor.authorGUERREIRO-FILHO, O.pt_BR
dc.contributor.authorMALUF, M. P.pt_BR
dc.date.accessioned2015-02-05T11:11:11Zpt_BR
dc.date.available2015-02-05T11:11:11Zpt_BR
dc.date.created2015-02-05pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationBMC Genomics, London, v. 15, p. 1-20, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1007801pt_BR
dc.descriptionA successful development of herbivorous insects into plant tissues depends on coordination of metabolic processes. Plants have evolved complex mechanisms to recognize such attacks, and to trigger a defense response. To understand the transcriptional basis of this response, we compare gene expression profiles of two coffee genotypes, susceptible and resistant to leaf miner (Leucoptera coffella). A total of 22000 EST sequences from the Coffee Genome Database were selected for a microarray analysis. Fluorescence probes were synthesized using mRNA from the infested and non-infested coffee plants. Array hybridization, scanning and data normalization were performed using Nimble Scan® e ArrayStar® platforms. Genes with foldchange values +/-2 were considered differentially expressed. A validation of 18 differentially expressed genes was performed in infected plants using qRT-PCR approach.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMicroarranjopt_BR
dc.subjectPlant defensept_BR
dc.titleLarge-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-02-06T11:11:11Zpt_BR
dc.subject.thesagroCafépt_BR
dc.subject.thesagroCoffea Arábicapt_BR
dc.subject.nalthesaurusLeafminerspt_BR
dc.subject.nalthesaurusGenespt_BR
dc.subject.nalthesaurusMicroarray technologypt_BR
riaa.ainfo.id1007801pt_BR
riaa.ainfo.lastupdate2015-02-06pt_BR
dc.identifier.doi10.1186/1471-2164-15-66pt_BR
dc.contributor.institutionDANIELLE C. CARDOSO, IAC; JULIANA C MARTINATI, IAC; POLIANA FERNANDA GIACHETTO, CNPTIA; RAMON O. VIDAL; MARCELO F. CARAZZOLLE; LILIAN PADILHA, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC; MIRIAN PEREZ MALUF, SAPC.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPTIA)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Largescaleanalysis.pdf2,13 MBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace