Título:	Development of genomic SSR markers and molecular characterization of Magnaporthe oryzae isolates from wheat in Brazil.
Autoria:	PEREIRA, J. F. CONSOLI, L. BOMBONATTO, E. A. de S. BONATO, A. L. V. MACIEL, J. L. N.
Afiliação:	JORGE FERNANDO PEREIRA, CNPT; LUCIANO CONSOLI, CNPT; ESTEVON ALEXANDRE DE SOUZA BOMBONATTO, ULBRA; ANA LIDIA VARIANI BONATO, CNPT; JOAO LEODATO NUNES MACIEL, CNPT.
Ano de publicação:	2014
Referência:	Biochemical Genetics, New York, v. 52, p. 52-70, 2014.
Conteúdo:	Magnaporthe oryzae, the causal agent of wheat blast, was characterized on a molecular level with 38 newly isolated genomic SSR loci. Among the 31 wheat isolates analyzed, 15 polymorphic loci were detected, with an average of 1.7 alleles per locus, 28.9% of them being highly or reasonably informative. The number of polymorphic loci was higher in isolates from Londrina in the Brazilian state of Parana´ and Coromandel in Minas Gerais compared with Goiânia in Goiás and São Borja in Rio Grande do Sul. The rice isolate was clearly different from the wheat isolates, and the size difference in polymorphic SSR loci between one isolate from wheat and one isolate from rice was associated with the number of repeats. Some isolates collected from different states and in different years demonstrated similarities of 100%. The markers developed here are useful for the genetic analysis of M. oryzae isolated from wheat, and isolates representing the variability detected in the field can be used to search for better wheat blast resistance.
Thesagro:	Trigo
ISSN:	0006-2928
Digital Object Identifier:	10.1007/s10528-013-9627-4
Notas:	Genetic variability Magnaporthe oryzae Microsatellite Wheat blast
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPT)