Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010483
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dc.contributor.authorRESENDE, J. A.pt_BR
dc.contributor.authorDINIZ, C. G.pt_BR
dc.contributor.authorSILVA, V. L.pt_BR
dc.contributor.authorOTENIO, M. H.pt_BR
dc.contributor.authorBONNAFOUS, A.pt_BR
dc.contributor.authorARCURI, P. B.pt_BR
dc.contributor.authorGODON, J.-J.pt_BR
dc.date.accessioned2015-03-10T01:49:57Z-
dc.date.available2015-03-10T01:49:57Z-
dc.date.created2015-03-03pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationJournal of Applied Microbiology, v. 117, n. 6, p. 1689-99, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1010483pt_BR
dc.descriptionAims: This study was focused on evaluating the persistency of antimicrobial resistance (AR) genes and putative pathogenic bacteria in an anaerobic digesters operating at mesophilic ambient temperature, in two different year seasons: summer and winter. Methods and Results: Abundance and dynamic of AR genes encoding resistance to macrolides (ermB), aminoglycosides (aphA2) and beta-lactams (blaTEM-1) and persistency of potentially pathogenic bacteria in pilot-scale anaerobic digesters were investigated. AR genes were determined in the influent and effluent in both conditions. Overall, after 60 days, reduction was observed for all evaluated genes. However, during the summer, anaerobic digestion was more related to the gene reduction as compared to winter. Persistency of potentially pathogenic bacteria was also evaluated by metagenomic analyses compared to an in-house created database. Clostridium, Acinetobacter and Stenotrophomonas were the most identified. Conclusions: Overall, considering the mesophilic ambient temperature during anaerobic digestion (summer and winter), a decrease in pathogenic bacteria detection through metagenomic analysis and AR genes is reported. Although the mesophilic anaerobic digestion has been efficient, the results may suggest medically important bacteria and AR genes persistency during the process. Significance and Impact of the Study: This is the first report to show AR gene dynamics and persistency of potentially pathogenic bacteria through metagenomic approach in cattle manure ambient temperature anaerobic digestion.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectAntibiotics resistance gene abundancept_BR
dc.subjectBacterial pathogenspt_BR
dc.subjectBiofertilizer dairy cattle manurept_BR
dc.subjectSeasonal variationspt_BR
dc.titleDynamics of antibiotic resistance genes and presence of putative pathogens during ambient temperature anaerobic digestion.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-03-10T01:49:57Zpt_BR
dc.subject.thesagroBiogáspt_BR
riaa.ainfo.id1010483pt_BR
riaa.ainfo.lastupdate2015-03-09pt_BR
dc.contributor.institutionJ. A. RESENDE, UFJFpt_BR
dc.contributor.institutionINRAeng
dc.contributor.institutionC. G. DINIZ, UFJFeng
dc.contributor.institutionV. L. SILVA, UFJFeng
dc.contributor.institutionMARCELO HENRIQUE OTENIO, CNPGLeng
dc.contributor.institutionA. BONNAFOUS, INRAeng
dc.contributor.institutionPEDRO BRAGA ARCURI, SRIeng
dc.contributor.institutionJ.-J. GODON, INRA.eng
Appears in Collections:Artigo em periódico indexado (CNPGL)

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