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dc.contributor.authorDIAS, N. L.pt_BR
dc.contributor.authorFONSECA JÚNIOR, A. A.pt_BR
dc.contributor.authorOLIVEIRA, A. M.pt_BR
dc.contributor.authorSALES, É. B.pt_BR
dc.contributor.authorALVES, B. R. C.pt_BR
dc.contributor.authorDORELLA, F. A.pt_BR
dc.contributor.authorCAMARGOS, M. F.pt_BR
dc.date.accessioned2015-03-03T11:11:11Zpt_BR
dc.date.available2015-03-03T11:11:11Zpt_BR
dc.date.created2015-03-03pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationVeterinary Medicine International, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1010526pt_BR
dc.descriptionThe viral disease classical swine fever (CSF), caused by a Pestivirus, is one of the major causes of economic losses for pig farming.The aim of this work was to validate a RT-qPCR using Taqman for detection of CSF in swine tissues. The parameters for the validation followed the specifications of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (OIE) and the guide ABNT NBR ISO/IEC 17025:2005. The analysis of the 5' NTR region of CSF virus was performed in 145 samples from 29 infected pigs and in 240 samples from 80 pigs originated in the Brazilian CSF-free zone. The tissues tested were spleen, kidney, blood, tonsils, and lymph nodes. Sequencing of the positive samples for 5'NTR region was performed to evaluate the specificity of the RT-qPCR. Tests performed for the RT-qPCR validation demonstrated that the PCR assay was efficient in detecting RNA from CSF virus in all materials from different tissues of infected animals. Furthermore, RNA from CSF virus was not detected in samples of swine originated from the Brazilian CSF-free zone. Hence, it is concluded that RT-qPCR can be used as a complementary diagnostic for CSF.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPCRpt_BR
dc.subjectSwine healthpt_BR
dc.subjectClassical Swine Feverpt_BR
dc.titleValidation of a real time PCR for classical swine fever diagnosis.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-03-03T11:11:11Zpt_BR
dc.format.extent24 p.pt_BR
riaa.ainfo.id1010526pt_BR
riaa.ainfo.lastupdate2015-03-03pt_BR
dc.contributor.institutionNATANAEL LAMAS DIAS, LANAGRO - MG; ANTÔNIO AUGUSTO FONSECA JÚNIOR, LANAGRO - MG; ANAPOLINO MACEDO OLIVEIRA, LANAGRO - MG; ÉRICA BRAVO SALES, LANAGRO - MG; BRUNA RIOS COELHO ALVES, CNPGL; FERNANDA ALVES DORELLA, LANAGRO - MG; MARCELO FERNANDES CAMARGOS, LANAGRO - MG.pt_BR
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