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dc.contributor.authorKOBORI, N. N.pt_BR
dc.contributor.authorMASCARIN, G. M.pt_BR
dc.contributor.authorJACKSON, M. A.pt_BR
dc.contributor.authorSCHISLER, D. A.pt_BR
dc.date.accessioned2017-08-09T23:47:32Z-
dc.date.available2017-08-09T23:47:32Z-
dc.date.created2015-03-19pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationFungal Biology, v. 119, n. 4, p. 179-190, Apr. 2015.pt_BR
dc.identifier.issn1878-6146pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1011851pt_BR
dc.descriptionMedia and culturing protocols were identified that supported the formation of submerged conidia and microsclerotia (MS) by Trichoderma harzianum Rifai strain T-22 using liquid culture fermentation. Liquid media with a higher carbon concentration (36 g L -1) promoted MS formation at all C:N ratios tested. Hyphae aggregated to form MS after 2 d growth and after 7 d MS were fully melanized. This is the first report of MS formation by T. harzianum or any species of Trichoderma. Furthermore, submerged conidia formation was induced by liquid culture media, but yields, desiccation tolerance, and storage stability varied with C:N ratio and carbon rate. Air-dried MS granules (<4 % moisture) retained excellent shelf life under cool and unrefrigerated storage conditions with no loss in conidial production. A low-cost complex nitrogen source based on cottonseed flour effectively supported high MS yields. Amending potting mix with dried MS formulations reduced or eliminated damping-off of melon seedlings caused by Rhizoctonia solani. Together, the results provide insights into the liquid culture production, stabilization process, and bioefficacy of the hitherto unreported MS of T. harzianum as a potential biofungicide for use in integrated management programs against soilborne diseases.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleLiquid culture production of microsclerotia and submerged conidia by Trichoderma harzianum active against damping-off disease caused by Rhizoctonia solani.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-08-09T23:47:32Zpt_BR
dc.subject.thesagroRhizoctonia solanipt_BR
dc.subject.thesagroTrichoderma harzianumpt_BR
dc.subject.nalthesaurusBiopesticidespt_BR
dc.subject.nalthesaurusSoil-borne diseasespt_BR
dc.subject.nalthesaurusSclerotiapt_BR
dc.subject.nalthesaurusCarbon nitrogen ratiopt_BR
riaa.ainfo.id1011851pt_BR
riaa.ainfo.lastupdate2017-08-09pt_BR
dc.identifier.doi10.1016/j.funbio.2014.12.005pt_BR
dc.contributor.institutionNILCE N. KOBORI, USDA; GABRIEL MOURA MASCARIN, CNPAF; MARK A. JACKSON, USDA; DAVID A. SCHISLER, USDA.pt_BR
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