Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1011872
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dc.contributor.authorSANTOS, A. S. de O. dospt_BR
dc.contributor.authorCOSTA, F. F.pt_BR
dc.contributor.authorESTEVES, W. T.pt_BR
dc.contributor.authorBRITO, M. A. V. P. ept_BR
dc.contributor.authorFURTADO, M. A. M.pt_BR
dc.contributor.authorMARTINS, M. F.pt_BR
dc.date.accessioned2015-03-20T11:11:11Zpt_BR
dc.date.available2015-03-20T11:11:11Zpt_BR
dc.date.created2015-03-20pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationRevista do Instituto de Laticínios Cândido Tostes, v. 69, n. 6, p. 415-423, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1011872pt_BR
dc.descriptionReliable methods for determination and quantification of total protein in food are essential information to ensure quality and safety of food trade. The objective of this study was to evaluate the linearity of calibration curves obtained from different proteins (blood serum albumin-BSA, α-LA, β-LG, caseins (CN): αs, β and κ-CAS) with the reagent of Bradford. Comercial UHT skimmed bovine milk was analyzed for the determination of total protein using the Bradford method by reading at 595 nm. The determination of the concentrations of total milk protein was achieved by linear regression. The Bradford method showed a high sensitivity for the determination of total proteins in bovine milk dilution 1:25 to values closer to those obtained by the Kjeldahl method. The results showed that the calibration curve of standard proteins β-CN and BSA obtained better linearity with less variation in the absorbance measurements for the determination of total protein of milk.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBovinept_BR
dc.subjectSkimmed milkpt_BR
dc.subjectSpectrophotometrypt_BR
dc.subjectProteins quantificationpt_BR
dc.subjectBSApt_BR
dc.titleLinearization of the Bradford protein assay to application in cow milk proteins quantification by UV-Vis spectrophotometry method.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-03-20T11:11:11Zpt_BR
dc.subject.nalthesaurusbeta-caseinpt_BR
riaa.ainfo.id1011872pt_BR
riaa.ainfo.lastupdate2015-03-20pt_BR
dc.identifier.doihttps://doi.org/10.14295/2238-6416.v69i6.350eng
dc.contributor.institutionALESSA SIQUEIRA DE OLIVEIRA DOS SANTOS; FABIANO FREIRE COSTA, UFJF; WESLEY TINOCO ESTEVES; MARIA APARECIDA V PAIVA E BRITO, CNPGL; MARCO ANTÔNIO MOREIRA FURTADO, UFJF; MARTA FONSECA MARTINS, CNPGL.pt_BR
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