Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013073
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dc.contributor.authorERBANO, M.pt_BR
dc.contributor.authorSCHUHLI, G. S. ept_BR
dc.contributor.authorSANTOS, É. P. dospt_BR
dc.contributor.otherMarianna Erbano, Pós-graduanda da UFPR; GUILHERME SCHNELL E SCHUHLI, CNPF; Élide Pereira dos Santos, UFPR.pt_BR
dc.date.accessioned2015-04-09T11:11:11Zpt_BR
dc.date.available2015-04-09T11:11:11Zpt_BR
dc.date.created2015-04-09pt_BR
dc.date.issued2015pt_BR
dc.identifier.other53594pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1013073pt_BR
dc.descriptionThe genetic diversity and population structure of Salvia lachnostachys Benth were assessed. Inter Simple Sequence Repeat (ISSR) molecular markers were used to investigate the restricted distribution of S. lachnostachys in Parana State, Brazil. Leaves of 73 individuals representing three populations were collected. DNA was extracted and submitted to PCR-ISSR amplification with nine tested primers. Genetic diversity parameters were evaluated. Our analysis indicated 95.6% polymorphic loci (stress value 0.02) with a 0.79 average Simpson?s index. The Nei-Li distance dendrogram and principal component analysis largely recovered the geographical origin of each sample. Four major clusters were recognized representing each collected population. Nei?s gene diversity and Shannon?s information index were 0.25 and 0.40 respectively. As is typical for outcrossing herbs, the majority of genetic variation occurred at the population level (81.76%). A high gene flow (Nm = 2.48) was observed with a correspondingly low fixation index. These values were generally similar to previous studies on congeneric species. The results of principal coordinate analysis (PCA) and of arithmetic average (UPGMA) were consistent and all three populations appear distinct as in STRUCTURE analysis. In addition, this analysis indicated a majority intrapopulation genetic variation. Despite the human pressure on natural populations our study found high levels of genetic diversity for S. lachnostachys. This was the first molecular assessment for this endemic species with medicinal proprieties and the results can guide for subsequent bioprospection, breeding programs or conservation actions.pt_BR
dc.description.uribitstream/item/122031/1/2015-API-GuilhermeS-GeneticVariability.pdfpt_BR
dc.languageenpt_BR
dc.language.isoengeng
dc.publisherInternational Journal of Molecular Sciences, v. 16, p. 7839-7850, 2015.pt_BR
dc.relation.ispartofEmbrapa Florestas - Artigo em periódico indexado (ALICE)pt_BR
dc.subjectSalvia lachnostachyspt_BR
dc.subjectVariabilidade genéticapt_BR
dc.subjectEstrutura populacionalpt_BR
dc.subjectMelhoramento genéticopt_BR
dc.subjectIntraspecific diversitypt_BR
dc.subjectISSRpt_BR
dc.subjectLabiatae.pt_BR
dc.titleGenetic variability and population structure of Salvia lachnostachys: implications for breeding and conservation programs.pt_BR
dc.typeArtigo em periódico indexado (ALICE)pt_BR
dc.date.updated2015-04-09T11:11:11Zpt_BR
dc.subject.thesagroConservação.pt_BR
dc.ainfo.id1013073pt_BR
dc.ainfo.lastupdate2015-04-09pt_BR
dc.identifier.doi10.3390/ijms16047839pt_BR
Appears in Collections:Artigo em periódico indexado (CNPF)

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