Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504
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dc.contributor.authorDORNELES, E. M. S.pt_BR
dc.contributor.authorSANTANA, J. A.pt_BR
dc.contributor.authorRIBEIRO, D.pt_BR
dc.contributor.authorDORELLA, F. A.pt_BR
dc.contributor.authorGUIMARAES, A. S.pt_BR
dc.contributor.authorMOAWAD, M. S.pt_BR
dc.contributor.authorSELIM, S. A.pt_BR
dc.contributor.authorGARALDI, A. L. M.pt_BR
dc.contributor.authorMIYOSHI, A.pt_BR
dc.contributor.authorRIBEIRO, M. G.pt_BR
dc.contributor.authorGOUVEIA, A. M. G.pt_BR
dc.contributor.authorAZEVEDO, V.pt_BR
dc.contributor.authorHEINEMANN, M. B.pt_BR
dc.contributor.authorLAGE, A. P.pt_BR
dc.date.accessioned2015-04-14T11:11:11Zpt_BR
dc.date.available2015-04-14T11:11:11Zpt_BR
dc.date.created2015-04-14pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationPlos One, v. 9, n. 6, e98758, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504pt_BR
dc.descriptionThe aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectEnterobacterial Repetitive Intergenic Consensus (ERIC-PCR)pt_BR
dc.titleEvaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-04-14T11:11:11Zpt_BR
riaa.ainfo.id1013504pt_BR
riaa.ainfo.lastupdate2015-04-14pt_BR
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0098758eng
dc.contributor.institutionELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG.pt_BR
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