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dc.contributor.authorAZEVEDO, H.pt_BR
dc.contributor.authorLOPES, F. M.pt_BR
dc.contributor.authorSILLA, P. R.pt_BR
dc.contributor.authorHUNGRIA, M.pt_BR
dc.date.accessioned2015-06-19T11:11:11Zpt_BR
dc.date.available2015-06-19T11:11:11Zpt_BR
dc.date.created2015-06-19pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationBMC Genomics, London, v. 16, 2015. Suppl 5, S10.pt_BR
dc.identifier.issn1471-2164pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1018160pt_BR
dc.descriptionBiological nitrogen fixation, with an emphasis on the legume-rhizobia symbiosis, is a key process for agriculture and the environment, allowing the replacement of nitrogen fertilizers, reducing water pollution by nitrate as well as emission of greenhouse gases. Soils contain numerous strains belonging to the bacterial genus Bradyrhizobium, which establish symbioses with a variety of legumes. However, due to the high conservation of Bradyrhizobium 16S rRNA genes - considered as the backbone of the taxonomy of prokaryotes - few species have been delineated. The multilocus sequence analysis (MLSA) methodology, which includes analysis of housekeeping genes, has been shown to be promising and powerful for defining bacterial species, and, in this study, it was applied to Bradyrhizobium, species, increasing our understanding of the diversity of nitrogen-fixing bacteria. Description: Classification of bacteria of agronomic importance is relevant to biodiversity, as well as to biotechnological manipulation to improve agricultural productivity. We propose the construction of an online database that will provide information and tools using MLSA to improve phylogenetic and taxonomic characterization of Bradyrhizobium, allowing the comparison of genomic sequences with those of type and representative strains of each species. Conclusion: A database for the taxonomic and phylogenetic identification of the Bradyrhizobium, genus, using MLSA, will facilitate the use of biological data available through an intuitive web interface. Sequences stored in the on-line database can be compared with multiple sequences of other strains with simplicity and agility through multiple alignment algorithms and computational routines integrated into the database. The proposed database and software tools are available at http://mlsa.cnpso.embrapa.br, and can be used, free of charge, by researchers worldwide to classify Bradyrhizobium, strains; the database and software can be applied to replicate the experiments presented in this study as well as to generate new experiments. The next step will be expansion of the database to include other rhizobial species.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleA database for the taxonomic and phylogenetic identification of the genus Bradyrhizobium using multilocus sequence analysis.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-05-11T11:11:11Zpt_BR
dc.subject.thesagroFixação de nitrogêniopt_BR
dc.subject.nalthesaurusNitrogen fixationpt_BR
dc.description.notesEdição dos Proceedings of the 10th International Conference of the Brazilian Association for Bioinformatics and Computational Biology (X-Meeting 2014), Belo Horizonte, Oct. 2014.pt_BR
dc.format.extent211 p.pt_BR
riaa.ainfo.id1018160pt_BR
riaa.ainfo.lastupdate2017-05-11pt_BR
dc.contributor.institutionHELTON DE AZEVEDO, CNPSO; Fabricio Martins Lopes; PAULO ROBERTO SILLA, CNPSO; MARIANGELA HUNGRIA DA CUNHA, CNPSO.pt_BR
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