Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1023363
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dc.contributor.authorFONSECA, F. C. de A.pt_BR
dc.contributor.authorFIRMINO, A. A. P.pt_BR
dc.contributor.authorMACEDO. L. L. P. dept_BR
dc.contributor.authorCOELHO, R. R.pt_BR
dc.contributor.authorSOUSA JÚNIOR, J. D. A. dept_BR
dc.contributor.authorSILVA JUNIOR, O. B.pt_BR
dc.contributor.authorTOGAWA, R. C.pt_BR
dc.contributor.authorPAPPAS JUNIOR, G. J.pt_BR
dc.contributor.authorGÓIS, L. A. B. dept_BR
dc.contributor.authorSILVA, M. C. M. dapt_BR
dc.contributor.authorSA, M. F. G. dept_BR
dc.contributor.otherFERNANDO CAMPOS DE ASSIS FONSECA, UnB; ALEXANDRE AUGUSTO PEREIRA FIRMINO, UnB; LEONARDO LIMA PEPINO DE MACEDO, UnB; ROBERTA RAMOS COELHO; JOSÉ DIJAIR ANTONINO DE SOUSA JÚNIOR, UnB; ORZENIL BONFIM SILVA-JUNIOR, UnB; ROBERTO COITI TOGAWA, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, Unb; LUIZ AVELAR BRANDÃO DE GÓIS, USINA TRUNFO, ALAGOAS; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN.pt_BR
dc.date.accessioned2015-09-04T11:11:11Zpt_BR
dc.date.available2015-09-04T11:11:11Zpt_BR
dc.date.created2015-09-04pt_BR
dc.date.issued2015pt_BR
dc.identifier.other35932pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1023363pt_BR
dc.descriptionSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control.pt_BR
dc.description.uribitstream/item/129096/1/journal.pone.0118231.pdfpt_BR
dc.languageenpt_BR
dc.language.isoengeng
dc.publisherPlos One, fev., 2015.pt_BR
dc.relation.ispartofEmbrapa Recursos Genéticos e Biotecnologia - Artigo em periódico indexado (ALICE)pt_BR
dc.titleSugarcane giant borer transcriptome analysis and identification of genes related to digestion.pt_BR
dc.typeArtigo em periódico indexado (ALICE)pt_BR
dc.date.updated2018-07-18T11:11:11Zpt_BR
dc.subject.thesagroBroca Gigantept_BR
dc.subject.thesagroControle Biológicopt_BR
dc.subject.thesagroPraga.pt_BR
dc.ainfo.id1023363pt_BR
dc.ainfo.lastupdate2018-07-18 -03:00:00pt_BR
dc.identifier.doi10.1371/journal.pone.0118231pt_BR
Appears in Collections:Artigo em periódico indexado (CENARGEN)

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