Title:	Characterization of rue extract and its potential for controlling rice blast.
Authors:	REIS, K. B. CORTÊS, M. V. de C. B. MARTINS, F. S. FILIPPI, M. C. C. de PAULA, J. R. de CONCEIÇÃO, E. C. da
Affiliation:	KARINNA BANNACH REIS, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; FREDERICO SEVERINO MARTINS, USP, Ribeirão Preto-SP; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; JOSE REALINO DE PAULA, UFG; EDEMILSON CARDOSO DA CONCEIÇÃO, UFG.
Date Issued:	2015
Citation:	Pesquisa Agropecuária Brasileira, v. 50, n. 12, p. 1121-1130, dez. 2015.
Description:	The objective of this work was to purify and standardize the extract of rue (Ruta graveolens) and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers furanocoumarins psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.
Thesagro:	Arroz Arruda Ruta graveolens Brusone
NAL Thesaurus:	Magnaporthe oryzae
Keywords:	Bergapteno Psoraleno Controle
DOI:	10.1590/S0100-204X2015001200001
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPAF)