Title:	Induction of neutralizing antibodies against mutalysin-II from Lachesis muta muta Snake Venom Elicited by a conformational B-cell epitope predicted by Blue Star Sting data base.
Authors:	MACHADO-DE-ÁVILA, R. A. VELLOSO, M. OLIVEIRA, D. STRANSKY, S. FLOR-SÁ, A. SCHNEIDER, F. S. NESHICH, G. CHÁVEZ-OLÓRTEGUI, C.
Affiliation:	RICARDO ANDREZ MACHADO-DE-ÁVILA, Universidade do Extremo Sul Catarinense, Criciúma-SC; MARIANA VELLOSO, UFMG; DAYSIANE OLIVEIRA, UFMG; STEPHANIE STRANSKY, UFMG; ANA FLOR-SÁ, UFMG; FRANCISCO S. SCHNEIDER, UFMG; GORAN NESHICH, CNPTIA; CARLOS CHÁVEZ-OLÓRTEGUI, Universidade do Extremo Sul Catarinense, Criciúma-SC.
Date Issued:	2015
Citation:	Immunome Research, v. 11, n. 1, Mar. 2015.
Pages:	Não paginado.
Description:	Mutalysin-II, from Lachesis muta muta snake venom, is an endopeptidase with hemorrhagic activity. To identify a conformational epitope we used Blue Star Sting, the latest version of the web based Sting Millenium Suite, as alternative computational analysis tool to select and design peptides. Pre-selected cut-off values of the accessibility and hydrophilicity parameters were used to select amino acid residues as potential conformational epitopes. A peptide (P117-Y116-C115-Q194-C195-L197-N198-K199-P200-Y5-L48) was manually drawn on Swiss-PDB-Viewer package and synthesized by Fmoc-synthesis. Immunization of rabbits with this peptide induced antibodies that recognized Mutalysin-II and protected against the hemorrhagic factors present in Lachesis venom. The Sting Millennium Suite was able to predict conformational epitopes in this class of proteins. Three amino acids (K199, Y5 and L48) were identified as essential in the interaction between the peptide and the neutralizing antibodies.
Thesagro:	Veneno
NAL Thesaurus:	Lachesis muta Antivenoms Synthetic peptides Epitopes
Keywords:	Peptídeos Sting millennium Conformational epitope prediction
DOI:	http://dx.doi.org/10.4172/1745-7580.1000083
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPTIA)