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dc.contributor.authorVOORT, M. van derpt_BR
dc.contributor.authorMEIJER, H. J. G.pt_BR
dc.contributor.authorSCHMIDT, Y.pt_BR
dc.contributor.authorWATROUS, Jpt_BR
dc.contributor.authorDEKKERS, E.pt_BR
dc.contributor.authorMENDES, R.pt_BR
dc.contributor.authorDORRESTEIN, P. Cpt_BR
dc.contributor.authorGROSS, H.pt_BR
dc.contributor.authorRAAIJMAKERS, J. M.pt_BR
dc.date.accessioned2016-01-04T11:11:11Zpt_BR
dc.date.available2016-01-04T11:11:11Zpt_BR
dc.date.created2016-01-04pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationFrontiers in Microbiology, Lausanne, v. 6, 2015. Article 696.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1032807pt_BR
dc.descriptionAbstract: The plant microbiome represents an enormous untapped resource for discovering novel genes and bioactive compounds. Previously, we isolated Pseudomonas sp. SH-C52 from the rhizosphere of sugar beet plants grown in a soil suppressive to the fungal pathogen Rhizoctonia solani and showed that its antifungal activity is, in part, attributed to the production of the chlorinated 9-amino-acid lipopeptide thanamycin (Mendes et al., 2011). To get more insight into its biosynthetic repertoire, the genome of Pseudomonas sp. SH-C52 was sequenced and subjected to in silico, mutational and functional analyses. The sequencing revealed a genome size of 6.3 Mb and 5579 predicted ORFs. Phylogenetic analysis placed strain SH-C52 within the Pseudomonas corrugata clade. In silico analysis for secondary metabolites revealed a total of six non-ribosomal peptide synthetase (NRPS) gene clusters, including the two previously described NRPS clusters for thanamycin and the 2-amino acid antibacterial lipopeptide brabantamide. Here we show that thanamycin also has activity and affects phospholipases of the late blight pathogen Phytophthora infestans. Most notably, mass spectrometry led to the discovery of a third lipopeptide, designated thanapeptin were found with varying degrees of activity against P. infestans. Of the remaining four NRPS clusters, one was predicted to encode for yet another and unknown lipopeptide with a predicted peptide moiety of 8-amino acids. Collectively, these results show an enormous metabolic potential for Pseudomonas sp. SH-C52, with at least three structurally diverse lipopeptides, each with a different antimicrobial activity spectrum.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPseudomonadspt_BR
dc.subjectGenomes sequencingpt_BR
dc.subjectBiocontrolpt_BR
dc.titleGenome mining and metabolic profiling of the rhizosphere bacterium Pseudomonas sp. SH-C52 for antimicrobial compounds.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-01-25T11:11:11Zpt_BR
dc.subject.thesagroRizosferapt_BR
dc.subject.thesagroBactériapt_BR
dc.subject.thesagroPseudomonas sppt_BR
dc.subject.thesagroPeptídeopt_BR
dc.subject.thesagroBeterrabapt_BR
dc.subject.thesagroControle biológicopt_BR
dc.subject.nalthesaurusBeneficial microorganismspt_BR
dc.subject.nalthesaurusRhizosphere bacteriapt_BR
dc.subject.nalthesaurusAntimicrobial peptidespt_BR
dc.subject.nalthesaurusSequence analysispt_BR
dc.subject.nalthesaurusMass spectrometrypt_BR
dc.subject.nalthesaurusBiological controlpt_BR
dc.format.extent214 p.pt_BR
riaa.ainfo.id1032807pt_BR
riaa.ainfo.lastupdate2016-01-25pt_BR
dc.contributor.institutionMENNO VAN DER VOORT, Wageningen University; HAROLD J G MEIJER, Wageningen University; YVONNE SCHMIDT, University of Bonn; JERAMIE WATROUS, University of California; ESTER DEKKERS, Wageningen University; RODRIGO MENDES, CNPMA; PIETER C DORRESTEIN, University of California; HARALD GROSS, University of California; JOS M RAAIJMAKERS, Wageningen University.pt_BR
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