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dc.contributor.authorGUIMARÃES, A. L. S.
dc.contributor.authorPEREIRA, S. A.
dc.contributor.authorKUSSANO, N. R.
dc.contributor.authorDODE, M. A. N.
dc.date.accessioned2018-09-05T00:34:51Z-
dc.date.available2018-09-05T00:34:51Z-
dc.date.created2016-01-06
dc.date.issued2015
dc.identifier.citationZygote, p. 1-11, 2015.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1033071-
dc.descriptionThis study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 M) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P < 0.05). When the cilostamide concentration was lowered (10 M) and oocytes were arrested for 8 h, embryo development was improved (P < 0.05) and was similar (P > 0.05) to the control. The deleterious effect of 20 M cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 M) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCultura pré-maturação
dc.subjectOócitos bovinos
dc.subjectCilostamide
dc.subjectMeiotic arrest
dc.titleThe effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes.
dc.typeArtigo de periódico
dc.date.updated2018-09-05T00:34:51Zpt_BR
riaa.ainfo.id1033071
riaa.ainfo.lastupdate2018-09-04
dc.identifier.doi10.1017/S0967199415000064
dc.contributor.institutionA. L. S. GUIMARÃES, UnB; S. A. PEREIRA, UnB; N. R. KUSSANO, UnB; MARGOT ALVES NUNES DODE, CENARGEN.
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