Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035434
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dc.contributor.authorCOUTO, E. G. de O.pt_BR
dc.contributor.authorVON PINHO, E. V. de R.pt_BR
dc.contributor.authorVON PINHO, R. G.pt_BR
dc.contributor.authorADRIANO DELLY VEIGApt_BR
dc.contributor.authorBUSTAMANTE, F. de O.pt_BR
dc.contributor.authorDIAS, K. O. das G.pt_BR
dc.date.accessioned2016-01-28T11:11:11Zpt_BR
dc.date.available2016-01-28T11:11:11Zpt_BR
dc.date.created2016-01-28pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationCiência e Agrotecnologia, v. 39, n. 5, Sept./Oct. 2015.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1035434pt_BR
dc.descriptionArtificial chromosome duplication is one of the most important process in the attainment of doubled haploids in maize. This study aimed to evaluate the induction ability of the inducer line KEMS in a tropical climate and test the efficiency of the R1-Navajo marker by flow cytometry to evaluate two chromosome duplication protocols and analyze the development of the doubled haploids in the field. To accomplish this goal, four genotypes (F1 and F2 generations) were crossed with the haploid inducer line KEMS. The seeds obtained were selected using the R1-Navajo marker and subject to two chromosome duplication protocols. Duplication was confirmed using flow cytometry. The percentages of self-fertilized plants after duplication as well as the quantities of doubled haploid seeds obtained after the self-fertilization processes were analyzed. It was observed that the germplasm influences haploid induction but not the duplication rates of the tested protocols. Protocol 2 was more efficient for the duplication of haploids, in the percentage of self-fertilized plants after duplication, and in the attainment of doubled haploid lines. Moreover, the haploid inducer line KEMS can produce haploids in a tropical climate. Other markers, in addition to the R1-Navajo system, should be used in the selection of haploid seeds.por
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectDuplicação cromossómicapt_BR
dc.subjectR-navajopt_BR
dc.subjectMaizept_BR
dc.subjectZea mays Lpor
dc.titleIn vivo haploid induction and efficiency of two chromosome duplication protocols in tropical maize.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-01-28T11:11:11Zpt_BR
dc.subject.thesagroMilhopt_BR
dc.subject.thesagroColchicinapt_BR
dc.description.notesTítulo em português: Indução in vivo de haploides e eficiência de dois protocolos de duplicação cromossômica em milho tropical.por
riaa.ainfo.id1035434pt_BR
riaa.ainfo.lastupdate2016-01-28pt_BR
dc.identifier.doihttps://doi.org/10.1590/S1413-70542015000500002por
dc.contributor.institutionEVELLYN GISELLY DE OLIVEIRA COUTO, UNIVERSIDADE FEDERAL DE LAVRASpt_BR
dc.contributor.institutionÉDILA VILELA DE RESENDE VON PINHO, UNIVERSIDADE FEDERAL DE LAVRASpor
dc.contributor.institutionRENZO GARCIA VON PINHO, UNIVERSIDADE FEDERAL DE LAVRASpor
dc.contributor.institutionVEIGA, A. D., CPACpor
dc.contributor.institutionFERNANDA DE OLIVEIRA BUSTAMANTE, UNIVERSIDADE FEDERAL DE LAVRASpor
dc.contributor.institutionKAIO OLIMPIO DAS GRAÇAS DIAS, UNIVERSIDADE FEDERAL DE LAVRAS.por
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