Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036144
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dc.contributor.authorMONTE, A. P. O. dopt_BR
dc.contributor.authorLOIOLA FILHO, J. B.pt_BR
dc.contributor.authorSOUZA, T. T. dos S.pt_BR
dc.contributor.authorMIRANDA, M. de S.pt_BR
dc.contributor.authorMAGALHÃES, L. C.pt_BR
dc.contributor.authorBARROS, C. H. S. C.pt_BR
dc.contributor.authorSILVA, A. A. de A.pt_BR
dc.contributor.authorSANTOS, A. O.pt_BR
dc.contributor.authorGUIMARÃES, A. de S. L.pt_BR
dc.contributor.authorCOSTA, J. M. da S.pt_BR
dc.contributor.authorSOUSA, P. H. F. dept_BR
dc.contributor.authorNOGUEIRA, D. M.pt_BR
dc.contributor.authorCORDEIRO, M. F.pt_BR
dc.contributor.authorLOPES JÚNIOR, E. S.pt_BR
dc.date.accessioned2016-02-04T11:11:11Zpt_BR
dc.date.available2016-02-04T11:11:11Zpt_BR
dc.date.created2016-02-04pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationSemina: Ciências Agrárias, Londrina, v. 36, n. 6, p. 4257-4268, 2015.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1036144pt_BR
dc.descriptionThis study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced in vivo. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was erformed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from ucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectRaça Dorperpt_BR
dc.subjectMúltipla ovulaçãoeng
dc.titleAddition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-03-29T11:11:11Zpt_BR
dc.subject.thesagroProdução animalpt_BR
dc.subject.thesagroOvinopt_BR
dc.subject.thesagroCriopreservaçãopt_BR
dc.subject.thesagroOvulaçãopt_BR
dc.subject.thesagroEmbrião Animalpt_BR
dc.subject.thesagroAçúcareng
dc.subject.thesagroOvelhaeng
dc.subject.nalthesaurusAnimal productionpt_BR
dc.subject.nalthesaurusSucrosept_BR
dc.subject.nalthesaurusCryopreservationeng
dc.subject.nalthesaurusEmbryo (animal)eng
dc.subject.nalthesaurusEweseng
dc.subject.nalthesaurusOvulationeng
riaa.ainfo.id1036144pt_BR
riaa.ainfo.lastupdate2016-03-29pt_BR
dc.identifier.doi10.5433/1679-0359.2015v36n6Supl2p4257pt_BR
dc.contributor.institutionALANE PAINS OLIVEIRA DO MONTE, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOpt_BR
dc.contributor.institutionJOÃO BOSCO LOIOLA FILHO, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOeng
dc.contributor.institutionTHAIS THATIANE DOS SANTOS SOUZA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOeng
dc.contributor.institutionMAYARA DE SOUZA MIRANDA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOeng
dc.contributor.institutionLÍVIA CORREIA MAGALHÃES, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOeng
dc.contributor.institutionCELSO HENRIQUE SOUZA COSTA BARROS, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOeng
dc.contributor.institutionANA ARLETE DE AMORIM SILVA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOeng
dc.contributor.institutionADRIANO OLIVEIRA SANTOS, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOeng
dc.contributor.institutionAIONNE DE SOUZA LEITE GUIMARÃES, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOeng
dc.contributor.institutionJONATHAN MAIA DA SILVA COSTA, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOeng
dc.contributor.institutionPEDRO HUMBERTO FÉLIX DE SOUSA, UNIVERSIDADE DO ESTADO DA BAHIAeng
dc.contributor.institutionDANIEL MAIA NOGUEIRA, CPATSAeng
dc.contributor.institutionMABEL FREITAS CORDEIRO, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCOeng
dc.contributor.institutionEDILSON SOARES LOPES JÚNIOR, UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO.eng
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