Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036889
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dc.contributor.authorARNOLD, D. R.pt_BR
dc.contributor.authorCORRÊA, C. A. P.pt_BR
dc.contributor.authorLORENA, L. L. G.pt_BR
dc.contributor.authorGASPAR, R. C.pt_BR
dc.contributor.authorROSSI, G. F.pt_BR
dc.contributor.authorIFRAM, A. M.pt_BR
dc.contributor.authorPENTEADO, J. C. T.pt_BR
dc.contributor.authorMINGOTI, G.pt_BR
dc.contributor.authorLOPES, F. L.pt_BR
dc.date.accessioned2016-02-15T11:11:11Zpt_BR
dc.date.available2016-02-15T11:11:11Zpt_BR
dc.date.created2016-02-15pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationPesquisa Veterinária Brasileira, Rio de janeiro, v. 35, n. 7, p. 605-612. jul. 2015.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1036889pt_BR
dc.descriptione aim of this study was to evaluate the histone modification, H3R26me2 during pre-implantation development of IVP bovine embryos cultured with or without serum supplementation and how these in vitro treatments compared to in vivo embryos at the morula stage. After in vitro maturation and fertilization, bovine embryos were cultured with either 0 or 2.5% fetal bovine serum (FBS). Development was evaluated and embryos were collected and fixed at different stages during development (2-, 4-, 8-, 16-cell, morula and blastocyst). Fixed embryos were then used for immunofluorescence utilizing an antibody for H3R26me2. Images of stained embryos were analyzed as a percentage of total DNA. Embryos cultured with 2.5% FBS developed to blastocysts at a greater rate than 0%FBS groups (34.85+5.43% vs. 23.38+2.93%; P<0.05). Levels of H3R26me2 changed for both groups over development. In the 0%FBS group, the greatest amount of H3R26me2 staining was at the 4-cell (P<0.05), 16-cell (P<0.05) and morula (P<0.05) stages. In the 2.5%FBS group, only 4-cell stage embryos were significantly higher than all other stages (P<0.01). Morula stage in vivo embryos had similar levels as the 0%FBS group, and both were signifcantly higher than the 2.5%FBS group. These results suggest that the histone modification H3R26me2 is regulated during development of pre-implantation bovine embryos, and that culture conditions greatly alter this regulation. INDEX TERMS: Bovine, embryo development,pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectH3R26me2pt_BR
dc.titleSupplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro proeduced bovine embryos.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-12-29T11:11:11Zpt_BR
dc.subject.nalthesaurusCattlept_BR
dc.subject.nalthesaurusEmbryogenesispt_BR
dc.subject.nalthesaurusFetal bovine serumpt_BR
dc.description.notesTítulo em português: Suplementação com soro fetal bovino altera a modificação de histona H3R26me2 durante o período pré-implantacional em embriões bovinos produzidos in vitro.pt_BR
riaa.ainfo.id1036889pt_BR
riaa.ainfo.lastupdate2017-12-29 -02:00:00pt_BR
dc.contributor.institutionDANIEL R. ARNOLD, USP; CAROLINA A. P. CORRÊA, UNESP; LAURA L. G. LORENA, UNESP; ROBERTA C. GASPAR, UNESP; GUILHERME F. ROSSI, UNESP; ADERSON M. IFRAN, UNESP; JOÃO C. T. PENTEADO, UNESP; GISELE MINGOTI, UNESP; FLAVIA L. LOPES, UNESP.pt_BR
Aparece nas coleções:Artigo em periódico indexado / Embrapa Unidades Centrais (AI-SEDE)

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