Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1039768
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorSANTOS, G. C.pt_BR
dc.contributor.authorPINHO, E. V. R. vonpt_BR
dc.contributor.authorROSA, S. D. V. F. dapt_BR
dc.date.accessioned2016-03-04T11:11:11Zpt_BR
dc.date.available2016-03-04T11:11:11Zpt_BR
dc.date.created2016-03-04pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationGenetics and Molecular Research, v. 12, n. 4, p6968-6982. 2013.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1039768pt_BR
dc.descriptionCoffee (Coffea arabica L.) seeds are sensitive to desiccation and oxidative stress during drying processes. We investigated the effect of drying and moisture levels on germination-related gene expressions associated with enzymatic systems that prevent oxidative stress in coffee seeds. Coffee seeds collected at physiological maturity were subjected to slow and quick drying to 40, 30, 20, and 12% moisture levels (wet basis), and as the control, seeds without drying were used. The seeds? physiological quality was calculated as percentage of normal seedlings at 15 and 30 days, normal vigorous seedlings at 30 days, and cotyledonary leaves at 45 days. The isoenzymes esterase, catalase (CAT), peroxidase (POX), and endo-?-mannanase expressions were electrophoretically analyzed. CAT and POX expressions were analyzed using RT-qPCR with specific primers constructed from the target gene sequences from the Brazilian Coffee Genome Database. Slow drying showed better physiological quality for seeds at 40 and 12% moisture levels, while quick drying was the most effective for seeds with 20% moisture. Sensitivity to water loss was confirmed by quick drying and activation of enzymes. CAT and POX transcriptions reduced during drying. RT-qPCR revealed a complex gene-expression pattern during the oxidative process, with high gene expression in wet seeds.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectDesiccation tolerancept_BR
dc.subjectEndo-?-mannanasept_BR
dc.subjectSeed agingpt_BR
dc.titleGene expression of coffee seed oxidation and germination processes during drying.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-03-04T11:11:11Zpt_BR
dc.subject.thesagroCafépt_BR
dc.subject.nalthesaurusIsozymespt_BR
riaa.ainfo.id1039768pt_BR
riaa.ainfo.lastupdate2016-03-04pt_BR
dc.contributor.institutionUFLA; UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC.pt_BR
Aparece nas coleções:Artigo em periódico indexado (SAPC)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Geneexpressionofcoffeeseed.pdf510,98 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace