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dc.contributor.authorALMEIDA, R. P. dept_BR
dc.contributor.authorSTOUTHAMER, R.pt_BR
dc.date.accessioned2016-03-23T06:46:14Z-
dc.date.available2016-03-23T06:46:14Z-
dc.date.created2016-03-22pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationBrazilian Journal of Biology, v. 75, n. 4, p. 974-982, 2015.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1041581pt_BR
dc.descriptionITS2 (Internal transcribed spacer 2) sequences have been used in systematic studies and proved to be useful in providing a reliable identification of Trichogramma species. DNAr sequences ranged in size from 379 to 632 bp. In eleven T. pretiosum lines Wolbachia-induced parthenogenesis was found for the first time. These thelytokous lines were collected in Peru (9), Colombia (1) and USA (1). A dichotomous key for species identification was built based on the size of the ITS2 PCR product and restriction analysis using three endonucleases (EcoRI, MseI and MaeI). This molecular technique was successfully used to distinguish among seventeen native/introduced Trichogramma species collected in South America.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMolecular markerspt_BR
dc.subjectEgg parasitoidpt_BR
dc.titleITS-2 sequences-based identification of Trichogramma species in South America.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-03-23T06:46:14Zpt_BR
dc.subject.thesagroMarcador molecularpt_BR
dc.subject.thesagroHimenopteropt_BR
dc.subject.thesagroTrichogramma sppt_BR
dc.subject.thesagroDNApt_BR
riaa.ainfo.id1041581pt_BR
riaa.ainfo.lastupdate2016-03-22pt_BR
dc.contributor.institutionRAUL PORFIRIO DE ALMEIDA, CNPA; R. STOUTHAMER, UNIVERSITY OF CALIFORNIA - USA.pt_BR
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