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dc.contributor.authorSANTOS, N. R.pt_BR
dc.contributor.authorALMEIDA, R. P. dept_BR
dc.contributor.authorPADILHA, I. Q. M.pt_BR
dc.contributor.authorARAÚJO, D. A. M.pt_BR
dc.contributor.authorCREÃO-DUARTE, A. J.pt_BR
dc.date.accessioned2016-03-22T11:11:11Zpt_BR
dc.date.available2016-03-22T11:11:11Zpt_BR
dc.date.created2016-03-22pt_BR
dc.date.issued2015pt_BR
dc.identifier.citationBrazilian Journal of Biology, v. 75, n. 2, p. 391-395, 2015.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1041586pt_BR
dc.descriptionThe objective of this work was the identification and differentiation of Trichogramma exiguum Pinto and Platner species, T. pretiosum Riley, and T. galloi Zucchi using sequences of the ITS2 region of ribosomal DNA. After extracting DNA from the studied species, a PCR reaction was performed, where the amplified samples were subjected to sequencing. The sequences obtained were submitted to a similarity search in GenBank (NCBI - National Center for Biotechnology Information) using the BLAST program, aiming to determine the similarity of these sequences with the species already deposited in the referenced database, and then multiple sequences were aligned using version 2.0 of the ClustalX program. According to the results of the multiple alignments of all sequences obtained, it was possible to observe the differences between the T. pretiosum, T. galloi and T. exiguum species. It was concluded that using the sequences of the ITS2 region of the ribosomal DNA was efficient in the differentiation of the studied Trichogramma species, which suggests a strong inter-specific variation among species.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMicrohymenopterapt_BR
dc.subjectParasitoidpt_BR
dc.subjectDNA ribossomalpt_BR
dc.titleMolecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-03-22T11:11:11Zpt_BR
dc.subject.thesagroDNApt_BR
dc.subject.nalthesaurusTrichogrammapt_BR
riaa.ainfo.id1041586pt_BR
riaa.ainfo.lastupdate2016-03-22pt_BR
dc.contributor.institutionN. R. SANTOS, UFPB; RAUL PORFIRIO DE ALMEIDA, CNPA; I. Q. M. PADILHA, UFPB; D. A. M. ARAÚJO, UFPB; A. J. CREÃO-DUARTE, UFPB.pt_BR
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