Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041600
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dc.contributor.authorPÉREZ-MONTAÑO, F.pt_BR
dc.contributor.authorCERRO, P. delpt_BR
dc.contributor.authorJIMÉNEZ-GUERRERO, I.pt_BR
dc.contributor.authorLÓPEZ-BAENA, F. J.pt_BR
dc.contributor.authorCUBO, M. T.pt_BR
dc.contributor.authorHUNGRIA, M.pt_BR
dc.contributor.authorMEGÍAS, M.pt_BR
dc.contributor.authorOLLERO, F. J.pt_BR
dc.date.accessioned2016-03-22T11:11:11Zpt_BR
dc.date.available2016-03-22T11:11:11Zpt_BR
dc.date.created2016-03-22pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationBMC Genomics, v. 17, n. 1, p. 198, Mar. 2016.pt_BR
dc.identifier.issn1471-2164pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1041600pt_BR
dc.descriptionRhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectRNA-seqpt_BR
dc.subjectNod factorspt_BR
dc.subjectLipochitooligosaccharidespt_BR
dc.subjectNodutlationpt_BR
dc.titleRNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-06-19T11:11:11Zpt_BR
dc.subject.thesagroNodulaçãopt_BR
dc.subject.thesagroRhizobiumpt_BR
dc.subject.nalthesaurusRhizobium tropicipt_BR
dc.subject.nalthesaurusApigeninpt_BR
dc.subject.nalthesaurusSalt stresspt_BR
riaa.ainfo.id1041600pt_BR
riaa.ainfo.lastupdate2017-06-19pt_BR
dc.identifier.doi10.1186/s12864-016-2543-3pt_BR
dc.contributor.institutionFrancisco Péres-Montaño, Universidade de Sevilla; Pablo del Cerro, Universidade de Sevilia; Irene Jiménez-Guerreiro, Universidade de Sevilla; Francisco Javier López-Baena, Universidade de Sevilla; maria Teresa Cubo, Universidade de Sevilla; MARIANGELA HUNGRIA DA CUNHA, CNPSO; Manuel Megías, Universidade de Sevilla; Francisco Javier Ollero, Uiversidade de Sevilla.pt_BR
Aparece nas coleções:Resumo em anais de congresso (CNPSO)

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