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dc.contributor.authorFALEIRO, F. G.pt_BR
dc.contributor.authorKANNAN, B.pt_BR
dc.contributor.authorALTPETER, F.pt_BR
dc.date.accessioned2017-08-10T23:56:05Z-
dc.date.available2017-08-10T23:56:05Z-
dc.date.created2016-04-11pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationPlant Cell, Tissue and Organ Culture, v. 124, n. 1, p. 57-67, January 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1043082pt_BR
dc.descriptionAbstract: Elephantgrass (Pennisetum purpureum, 2n = 4x = 28) produces large amounts of biomass in tropical and subtropical regions and is considered a prime candidate for lignocellulosic biofuel production. Interspecific hybridization between elephantgrass and pearl millet (Pennisetum glaucum, 2n = 2x = 14) may allow improvement of drought tolerance and biomass quality. These interspecific hybrids are male and female sterile due to their triploid genome (2n = 3x = 21). Chromosome doubling of the triploid hybrids may restore fertility, permitting a backcross with the recurrent or other elephantgrass parents to enhance biomass yield and persistence. In this study, chromosome doubling of productive interspecific hybrids was performed in vitro. Immature inflorescence cross-sections of five interspecific hybrids with good agronomic performance were used as explants for establishment of embryogenic tissue cultures. These calli were treated with different antimitotic agents, oryzalin or trifluralin. Chromosome doubling was achieved in all five interspecific hybrids and a total of 74 plants with altered ploidy were confirmed by flow cytometry. Stomatal size determination was a suitable screening tool for identification of hexaploid events. Genotypes MN18 and MN51 had the highest number of plants with altered ploidy of 29 and 27, respectively. Oryzalin at 5 µM was the most effective antimitotic treatment and produced 55 of the 74 plants with altered ploidy. The most vigorous lines were grown to maturity and produced viable pollen.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCitometria de fluxopt_BR
dc.subjectOrizalinapt_BR
dc.subjectTrifuralinapt_BR
dc.subjectDuplicação de cromossomopt_BR
dc.titleRegeneration of fertile, hexaploid, interspecific hybrids of elephantgrass and pearl millet following treatment of embryogenic calli with antimitotic agents.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-08-10T23:56:05Zpt_BR
dc.subject.thesagroCapim elefantept_BR
dc.subject.thesagroPlanta forrageirapt_BR
dc.subject.thesagroCultura de tecidopt_BR
dc.subject.thesagroEstômatopt_BR
dc.subject.thesagroCromossomapt_BR
dc.subject.thesagroMatéria primapt_BR
dc.subject.thesagroBiocombustívelpt_BR
dc.subject.thesagroPennisetum Purpureumpt_BR
dc.subject.nalthesaurusForagept_BR
dc.subject.nalthesaurusTissue culturept_BR
dc.subject.nalthesaurusStomatapt_BR
dc.subject.nalthesaurusChromosomespt_BR
dc.subject.nalthesaurusFeedstockspt_BR
dc.subject.nalthesaurusBiofuelspt_BR
riaa.ainfo.id1043082pt_BR
riaa.ainfo.lastupdate2017-08-10pt_BR
dc.identifier.doi10.1007/s11240-015-0874-4pt_BR
dc.contributor.institutionFABIO GELAPE FALEIRO, CPAC; BASKARAN KANNAN, UNIVERSITY OF FLORIDA; FREDY ALTPETER, UNIVERSITY OF FLORIDA.pt_BR
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