Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1043768
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dc.contributor.authorLACERDA, A. F.pt_BR
dc.contributor.authorSARTO, R. P. delpt_BR
dc.contributor.authorSILVA, M. S.pt_BR
dc.contributor.authorVASCONCELOS, E. A. R. dept_BR
dc.contributor.authorCOELHO, R. R.pt_BR
dc.contributor.authorSANTOS, V. O. dospt_BR
dc.contributor.authorGODOY, C. V.pt_BR
dc.contributor.authorSEIXAS, C. D. S.pt_BR
dc.contributor.authorSILVA, M. C. M. dapt_BR
dc.contributor.authorGROSSI-DE-SA, M. F.pt_BR
dc.date.accessioned2016-04-20T11:11:11Zpt_BR
dc.date.available2016-04-20T11:11:11Zpt_BR
dc.date.created2016-04-20pt_BR
dc.date.issued2016pt_BR
dc.identifier.citation3 Biotech, v. 6, n. 1, p. 59, Feb. 2016.pt_BR
dc.identifier.issn2190-5738pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1043768pt_BR
dc.descriptionPlant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian rust, as demonstrated by in planta assay. To our knowledge, this is the first report of a plant defensin active against an obligatory biotrophic phytopathogenic fungus. Results revealed the potential of rDrr230a as a candidate to be used in plant genetic engineering to control relevant cotton and soybean fungal diseases.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleThe recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium, Colletotrichum and Phakopsora.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-07-21T11:11:11Zpt_BR
dc.subject.thesagroSojapt_BR
dc.subject.thesagroAlgodãopt_BR
dc.subject.thesagroDoença de plantapt_BR
dc.subject.thesagroFungopt_BR
dc.subject.nalthesaurusSoybeanspt_BR
dc.subject.nalthesaurusCottonpt_BR
dc.subject.nalthesaurusPlant diseases and disorderspt_BR
dc.subject.nalthesaurusFungipt_BR
riaa.ainfo.id1043768pt_BR
riaa.ainfo.lastupdate2017-07-21pt_BR
dc.identifier.doi10.1007/s13205-015-0320-7pt_BR
dc.contributor.institutionARIANE FERREIRA LACERDA, Universidade Federal do Rio Grande do Norte; RAFAEL PERSEGHINI DEL SARTO, Centro Universitário do Distrito Federal; MARILIA SANTOS SILVA, Cenargen; ERICO AUGUSTO ROSAS DE VASCONCELOS; ROBERTA RAMOS COELHO; VANESSA OLINTO DOS SANTOS, Universidade Federal do Rio Grande do Norte; CLAUDIA VIEIRA GODOY, CNPSO; CLAUDINE DINALI SANTOS SEIXAS, CNPSO; MARIA CRISTINA MATTAR DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen.pt_BR
Appears in Collections:Artigo em periódico indexado (CNPSO)

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