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dc.contributor.authorCERRO, P. delpt_BR
dc.contributor.authorROLLA-SANTOS, A. A. P.pt_BR
dc.contributor.authorVALDERRAMA-FERNANDEZ, R.pt_BR
dc.contributor.authorGIL-SERRANO, A.pt_BR
dc.contributor.authorBELLOGÍN, R. A.pt_BR
dc.contributor.authorGOMES, D. F.pt_BR
dc.contributor.authorMONTAÑO, F. P.pt_BR
dc.contributor.authorMEGÍAS, M.pt_BR
dc.contributor.authorHUNGRIA, M.pt_BR
dc.contributor.authorJAVIER OLLERO, F.pt_BR
dc.date.accessioned2016-04-22T11:11:11Zpt_BR
dc.date.available2016-04-22T11:11:11Zpt_BR
dc.date.created2016-04-22pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationPLOS One, v. 11, n. 4, e0154029, Apr. 2016.pt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1043848pt_BR
dc.descriptionThe establishment of nitrogen-fixing rhizobium-legume symbioses requires a highly complex cascade of events. In this molecular dialogue the bacterial NodD transcriptional regulators in conjunction with plant inducers, mostly flavonoids, are responsible for the biosynthesis and secretion of Nod factors which are key molecules for successful nodulation. Other transcriptional regulators related to the symbiotic process have been identified in rhizobial genomes, including negative regulators such as NolR. Rhizobium tropici CIAT 899 is an important symbiont of common bean (Phaseolus vulgaris L.), and its genome encompasses intriguing features such as five copies of nodD genes, as well as other possible transcriptional regulators including the NolR protein. Here we describe and characterize a new regulatory gene located in the non-symbiotic plasmid pRtrCIAT899c, that shows homology (46% identity) with the nolR gene located in the chromosome of CIAT 899. The mutation of this gene, named nrcR (nolR-like plasmid c Regulator), enhanced motility and exopolysaccharide production in comparison to the wild-type strain. Interestingly, the number and decoration of Nod Factors produced by this mutant were higher than those detected in the wildtype strain, especially under salinity stress. The nrcR mutant showed delayed nodulation and reduced competitiveness with P. vulgaris, and reduction in nodule number and shoot dry weight in both P. vulgaris and Leucaena leucocephala. Moreover, the mutant exhibited reduced capacity to induce the nodC gene in comparison to the wild-type CIAT 899. The finding of a new nod-gene regulator located in a non-symbiotic plasmid may reveal the existence of even more complex mechanisms of regulation of nodulation genes in R. tropici CIAT 899 that may be applicable to other rhizobial species.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleNrcR, a new transcriptional regulator of Rhizobium tropici CIAT 899 involved in the Legume root-nodule symbiosis.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-07-10T11:11:11Zpt_BR
dc.subject.thesagroFixação de nitrogeniopt_BR
dc.subject.nalthesaurusNitrogen fixationpt_BR
riaa.ainfo.id1043848pt_BR
riaa.ainfo.lastupdate2017-07-10pt_BR
dc.identifier.doi10.1371/journal.pone.0154029pt_BR
dc.contributor.institutionPABLO DEL CERRO, UNIVERSIDAD DE SEVILLApt_BR
dc.contributor.institutionAMANDA A. P. ROLLA SANTOSeng
dc.contributor.institutionROCÍO VALDERRAMA FERNANDEZ, UNIVERSIDAD DE SEVILLAeng
dc.contributor.institutionANTONIO GIL SERRANO, UNIVERSIDAD DE SEVILLAeng
dc.contributor.institutionRAMÓN A. BELLOGÍN, UNIVERSIDAD DE SEVILLAeng
dc.contributor.institutionDOUGLAS FABIANO GOMES, UNIVERSIDADE ESTADUAL DE LONDRINAeng
dc.contributor.institutionFRANCISCO PÉREZ MONTAÑO, UNIVERSIDAD DE SEVILLAeng
dc.contributor.institutionMANUEL MEGÍAS, UNIVERSIDAD DE SEVILLAeng
dc.contributor.institutionMARIANGELA HUNGRIA DA CUNHA, CNPSOeng
dc.contributor.institutionFRANCISCO JAVIER OLLERO, UNIVERSIDAD DE SEVILLA.eng
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