Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1045048
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dc.contributor.authorDAMASCENO, J. C. A.pt_BR
dc.contributor.authorSOARES, A. C. F.pt_BR
dc.contributor.authorJESUS, F. N. dept_BR
dc.contributor.authorCASTRO, J. M. da C. ept_BR
dc.date.accessioned2016-05-16T11:11:11Zpt_BR
dc.date.available2016-05-16T11:11:11Zpt_BR
dc.date.created2016-05-16pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationNematoda, v. 3, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1045048pt_BR
dc.descriptionThe dyes used for staining nematode egg masses and eggs can cause environmental hazards and human health concerns. The aim of this study was to evaluate different artificial food dyes, as a replacement for acid fuchsin and phloxine B, for staining eggs and egg masses of Meloidogyne javanica from infected tomato plants. Five different combinations of dyes were tested at five concentrations. For control treatments, infected roots were stained with acid fuchsin and phloxine B for coloring eggs and egg masses, respectively. Egg staining with the dyes ponceau 4R with red 40 and ponceau 4R with brilliant blue, at the concentration of 4%, showed results similar to those obtained with acid fuchsin. However, egg masses were effectively stained with ponceau 4R food dyes with red 40 and with ponceau 4R with brilliant blue at all tested concentrations. Ponceau food coloring dyes gave a good contrast for nematode egg masses and egg counting, do not present human health and environmental pollution problems, and can be reused, with less waste being generated in the laboratory. These dyes are low-cost products that are available at most supermarkets, and can efficiently substitute acid fuchsin and phloxine B for staining eggs and egg masses of M. javanica.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectNematoide das galhaspt_BR
dc.subjectOvos de nematóidespt_BR
dc.subjectMassas de ovospt_BR
dc.subjectColoraçãopt_BR
dc.subjectCorantes alimentares artificiaispt_BR
dc.subjectInsectpt_BR
dc.titleRoot-knot nematode staining with artificial food dyes.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-02-16T11:11:11Zpt_BR
dc.subject.thesagroNematoidept_BR
dc.subject.thesagroInsetopt_BR
dc.subject.thesagroMeloidogyne Javanicapt_BR
riaa.ainfo.id1045048pt_BR
riaa.ainfo.lastupdate2017-02-16pt_BR
dc.identifier.doi10.4322/nematoda.01816pt_BR
dc.contributor.institutionJOSILDA CAVALCANTE AMORIM DAMASCENO, Universidade Federal do Recôncavo da Bahia - UFRB, Cruz das Almas BA; ANA CRISTINA FERMINO SOARES, Universidade Federal do Recôncavo da Bahia - UFRB, Cruz das Almas, BA; FÁBIO NASCIMENTO DE JESUS, Universidade Federal do Recôncavo da Bahia - UFRB, Cruz das Almas, BA; JOSE MAURO DA CUNHA E CASTRO, CPATSA.pt_BR
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