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dc.contributor.authorSANTOS, M. R. A. dospt_BR
dc.contributor.authorGUIMARÃES, M. C. M.pt_BR
dc.contributor.authorPAZ, E. S.pt_BR
dc.contributor.authorMAGALHÃES, G. M. O.pt_BR
dc.contributor.authorSOUZA, C. A.pt_BR
dc.contributor.authorSMOZINSKI, C. V.pt_BR
dc.contributor.authorNOGUEIRA, W. O.pt_BR
dc.date.accessioned2016-05-17T11:11:11Zpt_BR
dc.date.available2016-05-17T11:11:11Zpt_BR
dc.date.created2016-05-17pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationRevista Brasileira de Plantas Medicinais, Botucatu, v. 18, n. 1, p.142-148, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1045154pt_BR
dc.descriptionPiper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf?s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCallogenesispt_BR
dc.subjectReguladores de crescimentopt_BR
dc.titleInduction and growth pattern of callus from Piper permucronatum leaves.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-05-17T11:11:11Zpt_BR
dc.subject.thesagroCalogênesept_BR
dc.subject.thesagroPiperaceaept_BR
dc.subject.nalthesaurusgrowth regulatorspt_BR
riaa.ainfo.id1045154pt_BR
riaa.ainfo.lastupdate2016-05-17pt_BR
dc.contributor.institutionMAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia; UNIR; UNIR; UNIR; Bolsista, Embrapa Rondônia; Bolsista, Embrapa Rondônia; UNIR.pt_BR
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