Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1049759
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dc.contributor.authorMENEZES, K. A. S.pt_BR
dc.contributor.authorNUNES, G. F. de O.pt_BR
dc.contributor.authorSAMPAIO, A. A.pt_BR
dc.contributor.authorSILVA, A. F. dapt_BR
dc.contributor.authorSOUZA, L. S. B. dept_BR
dc.contributor.authorGAVA, C. A. T.pt_BR
dc.contributor.authorMARTINS, L. M. V.pt_BR
dc.contributor.authorFERNANDES JUNIOR, P. I.pt_BR
dc.date.accessioned2016-07-28T11:11:11Zpt_BR
dc.date.available2016-07-28T11:11:11Zpt_BR
dc.date.created2016-07-28pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationRevista de Ciências Agrárias, Lisboa, v. 39, n. 2, p. 222-233, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1049759pt_BR
dc.descriptionThis study aims to evaluate the phenotypical characteristics of bacterial isolates from mulungu (Erythrina velutina Willd.) nodules and determinate their Box-PCR fingerprinting. All bacteria were evaluated by the following phenotypic characteristics: growth rate, pH change, colony color and mucus production. The bacterial isolates able to re-nodulate the original host were also evaluated regarding its tolerance to increased salinity and different incubation temperatures, ability to growth using different carbon sources, intrinsic antibiotic resistance and ?in vitro? auxin biosynthesis. The molecular fingerprints were set up using the Box-PCR technique and the isolates were clustered by their profiles. Among the 22 bacterial isolates obtained, eight were able to re-nodulate the original host. Among the nodule inducing isolates, some were tolerant to 1% of NaCl and 39° C and all of them metabolized the maltose, fructose, glucose, sucrose and arabinose, were resistant to rifampicin and produced auxin. The bacteria showed low genetic similarity among them and reference strains, which indicates the great genetic variability of the isolates. The results of this work are the first reports about the bacterial isolates able to nodulate this species. A more deep study of these bacteria may reveal the existence of isolates tolerant to environmental stresses and suitable as a future mulungu inoculant.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBactérias isoladas de nódulospt_BR
dc.subjectBox-PCRpt_BR
dc.subjectFixação biológica de azotopt_BR
dc.subjectLeguminosa arbóreapt_BR
dc.subjectPlanta nativapt_BR
dc.subjectRizóbiopt_BR
dc.subjectBiological nitrogen fixationpt_BR
dc.subjectRhizobiapt_BR
dc.subjectInoculantpt_BR
dc.subjectTree legumespt_BR
dc.titleDiversity of new root nodule bacteria from Erythrina velutina Willd., a native legume from the Caatinga dry forest (Northeastern Brazil).pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-02-08T11:11:11Zpt_BR
dc.subject.thesagroCaatingapt_BR
dc.subject.thesagroMulungupt_BR
dc.subject.thesagroInoculantept_BR
dc.subject.nalthesaurusErythrina velutinapt_BR
riaa.ainfo.id1049759pt_BR
riaa.ainfo.lastupdate2017-02-08pt_BR
dc.identifier.doi10.19084/RCA15050pt_BR
dc.contributor.institutionKELLY ALEXSANDRA SOUZA MENEZES, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Juazeiro, BA; GERSIKA FAKIRRA DE OLIVEIRA NUNES, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Juazeiro, BA; ALINE ARAUJO SAMPAIO, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Juazeiro, BA; ALEKSANDRO FERREIRA DA SILVA, Universidade Federal Rural de Pernambuco; Recife, PE; LAYANE SILVA BARBOSA DE SOUZA, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Juazeiro, BA; CARLOS ALBERTO TUAO GAVA, CPATSA; LINDETE MÍRIA VIEIRA MARTINS, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Juazeiro, BA; PAULO IVAN FERNANDES JUNIOR, CPATSA.pt_BR
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