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dc.contributor.authorVIOL, M. A.pt_BR
dc.contributor.authorGUERRERO, F. D.pt_BR
dc.contributor.authorOLIVEIRA, B. C. M. dept_BR
dc.contributor.authorAQUINO, M. C. C. dept_BR
dc.contributor.authorLOIOLA, S. H.pt_BR
dc.contributor.authorMELO, G. D. dept_BR
dc.contributor.authorGOMES, A. H. de S.pt_BR
dc.contributor.authorKANAMURA, C. T.pt_BR
dc.contributor.authorGARCIA, M. V.pt_BR
dc.contributor.authorANDREOTTI, R.pt_BR
dc.contributor.authorLIMA, V. M. F. dept_BR
dc.contributor.authorBRESCIANI, K. D. S.pt_BR
dc.date.accessioned2016-08-02T11:11:11Zpt_BR
dc.date.available2016-08-02T11:11:11Zpt_BR
dc.date.created2016-08-02pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationParasitology Research, v. 115, n, 9, p. 3479-3484, sept. 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1050014pt_BR
dc.descriptionSand flies are recognized as the major vector of canine visceral leishmaniasis. However, in some areas of Brazil where sand flies do not occur, this disease is found in humans and dogs. There has been speculation that ticks might play a role in transmission of canine visceral leishmaniasis and the DNA of Leishmania spp. has been reported in whole ticks. We investigated the presence of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus ticks collected from tickinfested dogs in two cities of Brazil. We used 66 dogs that tested positive and 33 that tested negative for Leishmania spp. according to direct cytological examination assays. Ten ticks were collected from each dog and dissected to collect the intestines, ovaries, and salivary glands for immunohistochemistry (IHC) and diagnostic real-time polymerase chain reaction (RT-PCR). IHC results showed Leishmania spp. in 98, 14, and 8 % of the intestines, ovaries, and salivary glands, respectively. Real-time PCR showed that 89, 41, and 33 % of the tick intestine, ovary, and salivary glands, respectively, were positive for Leishmania spp. The verification of promastigotes of Leishmania spp. by two independent techniques in ticks collected from these urban region dogs showed that there is need for clarification of the role of ticks in the transmission of canine visceral leishmaniasis in Brazil.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectIxodidspt_BR
dc.titleIdentification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-03-28T11:11:11Zpt_BR
dc.subject.thesagroCãopt_BR
dc.subject.thesagroLeishmaniosept_BR
dc.subject.thesagroCarrapatopt_BR
dc.subject.nalthesaurusLeishmaniasispt_BR
dc.subject.nalthesaurusDogspt_BR
dc.subject.nalthesaurusImmunohistochemistrypt_BR
dc.subject.nalthesaurusQuantitative polymerase chain reactionpt_BR
riaa.ainfo.id1050014pt_BR
riaa.ainfo.lastupdate2017-03-28pt_BR
dc.contributor.institutionMilena Araúz Viol, UNESP; Felix D. Guerrero, UNESP; Bruno César Miranda de Oliveira, UNESP; Monally Conceição Costa de Aquino, UNESP; Saulo Hudson Loiola, UNESP; Guilherme Dias de Melo, UNESP; Aparecida Helena de Souza Gomes, Instituto Adolfo Lutz; Cristina Takami Kanamura, INSTITUTO ADOLFO LUTZ; Marcos Valério Garcia, FUNDECT; RENATO ANDREOTTI E SILVA, CNPGC; Valéria Marçal Félix de Lima, UNESP; Katia Denise Saraiva Bresciani, UNESP.pt_BR
Aparece en las colecciones:Artigo em periódico indexado (CNPGC)

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