Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1050864
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dc.contributor.authorSILVA, C. G.pt_BR
dc.contributor.authorMARTINS, C. F.pt_BR
dc.contributor.authorCARDOSO, T. C.pt_BR
dc.contributor.authorCUNHA, E. R. dapt_BR
dc.contributor.authorCUMPA, H. C. B.pt_BR
dc.contributor.authorMARTINS, G. H. L.pt_BR
dc.contributor.authorPIVATO, I.pt_BR
dc.contributor.authorBÁO S. N.pt_BR
dc.date.accessioned2016-08-15T11:11:11Zpt_BR
dc.date.available2016-08-15T11:11:11Zpt_BR
dc.date.created2016-08-15pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationCellular Reprogramming, v. 18, n. 2, p. 127-136, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1050864pt_BR
dc.descriptionThe less differentiated the donor cells are used in nuclear transfer (NT), the more easily are they reprogrammed by the recipient cytoplasm. In this context, mesenchymal stem cells (MSCs) appear as an alternative to donor nuclei for NT. The amniotic fluid and adipose tissue are sources of MSCs that have not been tested for the production of cloned embryos in cattle. The objective of this study was to isolate, characterize, and use MSCs derived from amniotic fluid (MSC-AF) and adipose tissue (MSC-AT) to produce cloned calves. Isolation of MSC-AF was performed using in vivo ultrasound-guided transvaginal amniocentesis, and MSC-AT were isolated by explant culture. Cellular phenotypic and genotypic characterization by flow cytometry, immunohistochemistry, and RT-PCR were performed, as well as induction in different cell lineages. The NT was performed using MSC-AF and MSC-AT as nuclear donors. The mesenchymal markers of MSC were expressed in bovine MSC-AF and MSC-AT cultures, as evidenced by flow cytometry, immunohistochemistry, and RT-PCR. When induced, these cells differentiated into osteocytes, chondrocytes, and adipocytes. Embryo production was similar between the cell types, and two calves were born. The calf from MSC-AT was born healthy, and this fact opens a new possibility of using this type of cell to produce cloned cattle by NT.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCélulas estaminais mesenquimaispt_BR
dc.subjectLíquido aminióticopt_BR
dc.subjectTecido adiposopt_BR
dc.subjectProdução de embriãopt_BR
dc.titleProduction of bovine embryos and calves cloned by nuclear transfer using mesenchymal stem cells from amniotic fluid and adipose tissue.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-09-08T11:11:11Zpt_BR
dc.subject.thesagroBezerropt_BR
dc.subject.thesagroBovinopt_BR
dc.subject.thesagroClonept_BR
dc.subject.nalthesaurusStem cellseng
dc.subject.nalthesaurusAmniotic fluideng
dc.subject.nalthesaurusEmbryo (animal)eng
dc.subject.nalthesaurusAdipose tissueeng
dc.format.extent2p. 127-136pt_BR
riaa.ainfo.id1050864pt_BR
riaa.ainfo.lastupdate2016-09-08pt_BR
dc.identifier.doihttps://doi.org/10.1089/cell.2015.0064eng
dc.contributor.institutionCAROLINA GONZALES SILVA, UNIVERSIDADE DE BRASÍLIApt_BR
dc.contributor.institutionCARLOS FREDERICO MARTINS, CPACeng
dc.contributor.institutionTEREZA CRISTINA CARDOSO, UNIVERSIDADE DE SÃO PAULOeng
dc.contributor.institutionELISA RIBEIRO DA CUNHA, UNIVERSIDADE DE BRASÍLIAeng
dc.contributor.institutionHEIDI CHRISTINA BESSLER CUMPA, CPACeng
dc.contributor.institutionGEORGE HENRIQUE LIMA MARTINSeng
dc.contributor.institutionIVO PIVATO, UNIVERSIDADE DE BRASÍLIAeng
dc.contributor.institutionSÔNIA NAIR BÁO, UNIVERSIDADE DE BRASÍLIA.eng
Appears in Collections:Artigo em periódico indexado (CPAC)

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