Título:	Molecular detection of Erwinia psidii in guava plants under greenhouse and field conditions.
Autor:	SILVA, C. F. da UESUGI, C. H. BLUM, L. E. B. MARQUES, A. S. A. FERREIRA, M. A. da S. V.
Afiliación:	CLAUDÊNIA FERREIRA DA SILVA, UNB; CARLOS HIDEMI UESUGI, UNB; LUIZ EDUARDO BASSAY BLUM, UNB; ABI SOARES DOS ANJOS MARQUES, SIQ; MARISA ÁLVARES DA SILVA VELLOSO FERREIRA, UNB.
Año:	2016
Referencia:	Ciência Rural, Santa Maria, v. 46, n. 9, p. 1528-1534, 2016.
Descripción:	Erwinia psidii causes bacterial blight of guava (Psidium guajava ), an important disease of this crop in Brazil. The pathogen affects branches and twigs of guava trees, reducing yield significantly. Bacterial dissemination often occurs through contaminated but asymptomatic propagating plant material. The objectives of this research were to evaluate the use of BIO-PCR and conventional PCR to detectE. psidii in inoculated guava plants grown in a greenhouse and in symptomatic and asymptomatic trees from guava orchards.Erwinia psidii strain IBSBF 1576 was inoculated (107CFU mL-1) into young guava shoots and plant tissue was analysed at 0, 5, 10, and 15 days after inoculation. Symptoms were observed after 5 days and all inoculated shoots were PCR positive at all times, by both BIO-PCR and conventional PCR. Under natural infection conditions, 40 samples were tested by BIO-PCR from each of three guava orchards, 20 showing symptoms and 20 asymptomatic. PCR was positive for 58 out of 60 symptomatic samples (96.7%) and for 6.7% of asymptomatic samples, showing that the method can be used to detect the pathogen at early stages of infection. This PCR method may be used as a diagnostic tool to assess bacterial survival, dissemination and disease outbreaks.
Thesagro:	Seca dos ponteiros Psidium Guajava
Palabras clave:	BIO-PCR Diagnose PCR Diagnosis Guava bacterial blight
DOI:	org/10.1590/0103-8478cr20151600
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CENARGEN)