Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053027
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dc.contributor.authorPAIS, A. K.pt_BR
dc.contributor.authorSILVA, A. P. dapt_BR
dc.contributor.authorSOUZA, J. C. dept_BR
dc.contributor.authorTEIXEIRA, S. L.pt_BR
dc.contributor.authorRIBEIRO, J. M.pt_BR
dc.contributor.authorPEIXOTO, A. R.pt_BR
dc.contributor.authorPAZ, C. D. dapt_BR
dc.date.accessioned2016-09-19T11:11:11Zpt_BR
dc.date.available2016-09-19T11:11:11Zpt_BR
dc.date.created2016-09-19pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationAfrican Journal of Biotechnology, v. 15, n. 36, p. 1995-1998, sept. 2016pt_BR
dc.identifier.issn1684-5315pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1053027pt_BR
dc.descriptionMicropropagation requires controlling contamination that might compromise the success of the process. Thermal sterilization is traditionally used; however, costs deriving from equipment acquisition and maintenance render this technique costly. With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for sterilization of culture media and glassware used during rooting of micropropagated Gerbera hybrida cv. Essandre. Two experiments were carried out. In the first one, treatments consisted of control I (no sterilization), control II (thermal sterilization), and total active chlorine concentrations of 0.0005, 0.001, 0.002 and 0.003%. In the second experiment, based on the results observed in the first experiment, treatments consisted of control I (thermal sterilization) and II (chemical sterilization), and total active chlorine concentrations of 0.002, 0.0025 and 0.003%. Plant behavior was assessed based on the length of aerial part and roots, number of roots, and dry biomass of plants. Results showed that the addition of an active chlorine concentration of 0.003% to culture media provided total control of contaminants, and there were no significant differences regarding the variables analyzed between plants obtained with thermal sterilization and with sodium hypochlorite sterilization. Thus, chemical sterilization can be used as a replacement for thermal sterilization of nutrition media for rooting of gerbera in vitro.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectNaOCpt_BR
dc.subjectHipoclorito de sódiopt_BR
dc.subjectEsterilização químicapt_BR
dc.subjectAutoclavagempt_BR
dc.subjectIn vitropt_BR
dc.subjectChemical sterilizationpt_BR
dc.subjectContaminationpt_BR
dc.titleSodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-09-22T11:11:11Zpt_BR
dc.subject.thesagroCultura de tecidopt_BR
dc.subject.thesagroGerberapt_BR
dc.subject.thesagroPlanta ornamentalpt_BR
dc.subject.thesagroMicropropagaçãopt_BR
dc.subject.thesagroContaminaçãopt_BR
dc.subject.nalthesaurusSodium hypochloritept_BR
dc.subject.nalthesaurusTissue culturept_BR
riaa.ainfo.id1053027pt_BR
riaa.ainfo.lastupdate2016-09-22pt_BR
dc.identifier.doi10.5897/AJB2016.15405pt_BR
dc.contributor.institutionANA KAROLINA PAIS, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia – UNEB; ANA PAULA DA SILVA, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia – UNEB; JOSELITA CARDOSO DE SOUZA, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia – UNEB; SILVIO LOPES TEIXEIRA; JULIANA MARTINS RIBEIRO, CPATSA; ANA ROSA PEIXOTO, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia – UNEB; CRISTIANE DOMINGOS DA PAZ, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia – UNEB.pt_BR
Appears in Collections:Artigo em periódico indexado (CPATSA)

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