Dedifferentiation of Leaf Cells and Growth Pattern of Calluses of Capsicum annuumcv. Etna.

SANTOS, M. R. A. dos; SOUZA, C. A. de

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Campo DC	Valor	Idioma
dc.contributor.author	SANTOS, M. R. A. dos	pt_BR
dc.contributor.author	SOUZA, C. A. de	pt_BR
dc.date.accessioned	2016-09-22T11:11:11Z	pt_BR
dc.date.available	2016-09-22T11:11:11Z	pt_BR
dc.date.created	2016-09-22	pt_BR
dc.date.issued	2016	pt_BR
dc.identifier.citation	Australian Journal of Basic and Applied Sciences, v. 10, n. 12, p. 362-368, 2016.	pt_BR
dc.identifier.uri	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053310	pt_BR
dc.description	Background:In vitrocell suspension cultivation systems have been largely reported assafe and standardized methods for production of secondary metabolites with medicinaland agricultural interest.Capsicum annuumis one of the most widely grown vegetablein the world and its biological activities have been demonstrated against insects, fungi,bacteria and other groups of organisms. The determination of procedures for thededifferentiation of cells into callus cells and the subsequent study of the callus growthpattern are necessary for the establishment of cellsuspensions and also to subsidizestudies regarding the bioactivity of its secondarymetabolites. To date, no study hasdescribed the development of protocols for callus induction inC. annuumL. cv. Etna. Objective:The objective of this study was to establish a protocol for dedifferentiationof leaf cells of the cultivarC. annuumcv. Etna and to determine the growth pattern ofthe calluses with a focus on the deceleration phase, when the callus cells must besubcultured into a liquid medium in order to establish cell suspension cultivationsaiming at the production of secondary metabolites.Results:The treatment that resultedin the highest %CI, ACCC and callus weight was thecombination of 4.52 μ M 2,4-D +0.44 μ M BA. The calluses produced were friable andwhitish and their growth patternfollowed a sigmoid shape. The deceleration phase started on the 23rdday of cultivation.Conclusion:Callus induction in leaf explants ofC. annuumcv. Etnacan be achieved inMS medium supplemented with 4.52 μ M 2,4-D + 0.44 μ MBA, which results in highcellular proliferation; in order to start a cell suspension culture, callus cells on the 23rdday of culture should be used.	pt_BR
dc.language.iso	eng	eng
dc.rights	openAccess	eng
dc.subject	Callogenesis	pt_BR
dc.subject	Growth curve	pt_BR
dc.subject	Metabolismo secundário	pt_BR
dc.subject	Calogêneses	pt_BR
dc.title	Dedifferentiation of Leaf Cells and Growth Pattern of Calluses of Capsicum annuumcv. Etna.	pt_BR
dc.type	Artigo de periódico	pt_BR
dc.date.updated	2016-10-06T11:11:11Z	pt_BR
dc.subject.thesagro	Curva de Crescimento	pt_BR
dc.subject.thesagro	Pimenta	pt_BR
dc.subject.nalthesaurus	secondary metabolites	pt_BR
riaa.ainfo.id	1053310	pt_BR
riaa.ainfo.lastupdate	2016-10-06	pt_BR
dc.contributor.institution	MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia; Carolina Augusto de Souza.	pt_BR
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