Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1054691
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dc.contributor.authorABREU, F. R. M.pt_BR
dc.contributor.authorDEUS, K. E. dept_BR
dc.contributor.authorPEREIRA, W. J.pt_BR
dc.contributor.authorSILVEIRA, R. D. D.pt_BR
dc.contributor.authorVIANELLO, R. P.pt_BR
dc.contributor.authorBRONDANI, C.pt_BR
dc.date.accessioned2016-10-14T11:11:11Zpt_BR
dc.date.available2016-10-14T11:11:11Zpt_BR
dc.date.created2016-10-14pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationAustralian Journal of Crop Science, v. 10, n. 9, p. 1266-1271, 2016.pt_BR
dc.identifier.issn1835-2707pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1054691pt_BR
dc.descriptionThe identification and validation of candidate genes related to traits of interest is a time consuming and expensive process and the homology among genes from different species can facilitate the identification of genes of the target species from the genomic information of a model species. This study aimed to quantify the expression of homologous rice genes previously related to drought tolerance in Arabidopsis. Five genes (CPK6, PLDa, GluR2, CesA8, and EIN2) were identified in rice by the homology of the amino acid sequence between rice and Arabidopsis. The genotypes Douradão (drought tolerant) and Primavera (drought susceptible) were subjected to a water deficit experiment, and subsequently evaluated for gene expression by qPCR for the five homologous and Lsi1 genes. The qPCR analysis clearly showed that the five homologous genes were expressed in rice, which is an indication that these genes could preserve their function in rice as a response to drought. In Douradão, of the five homologous genes, all but OsGluR2 displayed an increase in the average expression in drought treatment when compared to the control, while in Primavera, the average expression of the five genes did not differ between the control and drought treatment. In Douradão, the OsPLDa1, which showed the higher expression level in drought in relation to the control (10.82), significantly increased the gene expression in the leaf and root tissues as a response to drought, in both vegetative and reproductive stages, whereas in Primavera, this gene was suppressed in both tissues and stages under drought. Therefore, the OsPLDa1 gene was the most important in relation to drought response and is an interesting candidate for further studies in developing rice cultivars that are more tolerant to this stress.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGene homologypt_BR
dc.titleExpression of rice genes homologous of Arabidopsis genes previously related to drought tolerance.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-03-03T11:11:11Zpt_BR
dc.subject.thesagroArrozpt_BR
dc.subject.thesagroOryza sativapt_BR
dc.subject.thesagroAcido abscisicopt_BR
dc.subject.thesagroRendimentopt_BR
dc.subject.nalthesaurusRicept_BR
dc.subject.nalthesaurusAbscisic acidpt_BR
dc.subject.nalthesaurusQuantitative polymerase chain reactionpt_BR
dc.subject.nalthesaurusYieldspt_BR
dc.subject.nalthesaurusArabidopsispt_BR
riaa.ainfo.id1054691pt_BR
riaa.ainfo.lastupdate2017-03-03pt_BR
dc.contributor.institutionFERNANDA RAQUEL MARTINS ABREU, UNIVERSIDADE FEDERAL DE GOIÁS; KARINE EVARISTO DE DEUS, UNIVERSIDADE FEDERAL DE GOIÁS; WENDELL JACINTO PEREIRA, UNIVERSIDADE FEDERAL DE GOIÁS; RICARDO DIOGENES DIAS SILVEIRA; ROSANA PEREIRA VIANELLO, CNPAF; CLAUDIO BRONDANI, CNPAF.pt_BR
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