Título:	Iron content affects lipogenic gene expression in the muscle of nelore beef cattle.
Autoria:	DINIZ, W. J. da S. COUTINHO, L. L. TIZIOTO, P. C. CESAR, A. S. M. GROMBONI, C. F. NOGUEIRA, A. R. de A. OLIVEIRA, P. S. N. de SOUZA, M. M. de REGITANO, L. C. de A.
Afiliação:	WELLISON JARLES DA SILVA DINIZ, UFSCAR; LUIZ LEHMANN COUTINHO, USP; POLYANA CRISTINE TIZIOTO, FAPESP; ALINE SILVA MELLO CESAR, USP/ESALQ; CAIO FERNANDO GROMBONI, UFBA; ANA RITA DE ARAUJO NOGUEIRA, CPPSE; PRISCILA SILVA NEUBERN DE OLIVEIRA, FAPESP; MARCELA MARIA DE SOUZA, UFSCAR; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Ano de publicação:	2016
Referência:	Plos One, aug. 2016.
Conteúdo:	Iron (Fe) is an essential mineral for metabolism and plays a central role in a range of biochemical processes. Therefore, this study aimed to identify differentially expressed (DE) genes and metabolic pathways in Longissimus dorsi (LD) muscle from cattle with divergent iron content, as well as to investigate the likely role of these DE genes in biological processes underlying beef quality parameters. Samples for RNA extraction for sequencing and iron, copper, manganese, and zinc determination were collected from LD muscles at slaughter. Eight Nelore steers, with extreme genomic estimated breeding values for iron content (Fe-GEBV), were selected from a reference population of 373 animals. From the 49 annotated DE genes (FDR<0.05) found between the two groups, 18 were upregulated and 31 down-regulated for the animals in the low Fe-GEBV group. The functional enrichment analyses identified several biological processes, such as lipid transport and metabolism, and cell growth. Lipid metabolism was the main pathway observed in the analysis of metabolic and canonical signaling pathways for the genes identified as DE, including the genes FASN, FABP4, and THRSP, which are functional candidates for beef quality, suggesting reduced lipogenic activities with lower iron content. Our results indicate metabolic pathways that are partially influenced by iron, contributing to a better understanding of its participation in skeletal muscle physiology.
Thesagro:	Gado Nelore Ferro
NAL Thesaurus:	Nellore
Palavras-chave:	Fisiologia esquelético muscular Metabolismo lipídico
Digital Object Identifier:	http://dx.doi.org/10.1371/journal.pone.0161160
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPPSE)