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dc.contributor.authorSANTOS, M. R. A. dospt_BR
dc.contributor.authorPAZ, E. S.pt_BR
dc.date.accessioned2016-11-29T11:11:11Zpt_BR
dc.date.available2016-11-29T11:11:11Zpt_BR
dc.date.created2016-11-29pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationInternational Journal of Current Research, v. 8 , n. 9, p. 38688 - 38691, Set., 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1057485pt_BR
dc.descriptionThe objective of this research was to determine the effect of dichlorophenoxyacetic acid (2,4-D) callus in peach friable calluses in leaf explants of Bactris gasipaes H.B.K., aiming for further induction of somatic embryos. The explants were inoculated in MS medium supplemented with 30.0 g.L-1 sucrose, 8.0 g.L-1 agar and 2,4-D (0.0, 0.31, 0.62, 1.25, 2.5, 5.0, 10.0, and 20.0 mg.L-1). The cultures were kept in a growth room at 24±2°C under light conditions (50 µmol.m-2.s-1, photoperiod of 16 hours), and also under dark conditions, in factorial arrangement: 2 (light and dark conditions) x 8 (2,4-D concentrations), totaling 16 treatments. On the 14th day of cultivation, occurrences of oxidation and necrosis of the explants were observed and, on the 35th day, the formation of friable calluses, and the percentage of explant area covered by callus cells (EACC) were evaluated. Oxidation was more intense in the cultivations under light conditions, and reduced as the 2,4-D concentrationsincreased. However, the highest concentration, 20.0 mg.L-1, caused necrosis of the explants. The concentration of 10 mg.L-1 was the most efficient for friable callus induction reaching all the explants in the cultures kept in the dark. This concentration also resulted in the highest EACC, with an average of 56.5% of the explant area covered by callus cells.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPeach palmpt_BR
dc.subjectCallogenesispt_BR
dc.subjectDichlorophenoxyacetic acidpt_BR
dc.subjectPessegueiropt_BR
dc.subjectÁcido diclorofenoxiacéticopt_BR
dc.titleEffect of 2,4-d on callus induction in leaf explants of peach palm (bactris gasipaes h.b.k.).pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-11-29T11:11:11Zpt_BR
dc.subject.thesagroCalogênesept_BR
dc.subject.nalthesaurusArecaceaept_BR
riaa.ainfo.id1057485pt_BR
riaa.ainfo.lastupdate2016-11-29pt_BR
dc.contributor.institutionMAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia; Eloísa Santana Paz, UNIR.pt_BR
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