Title:	Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
Authors:	NASCIMENTO, A. R. SILVA, G. F. GUALBERTO, G. F. ALMEIDA, F. L.
Affiliation:	A. R. Nascimento, Faculdade Estácio do Amazonas; GILVAN FERREIRA DA SILVA, CPAA; G. F. Gualberto; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA.
Date Issued:	2016
Citation:	Genetics and Molecular Research, v. 15, n. 4, p. 15049228, 2016.
Description:	Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of reference genes for the relative or absolute quantification of gene transcripts, we cloned and sequenced three candidate reference genes in tambaqui, and validated a set of primers for each gene for use in real-time quantitative PCR.
Thesagro:	Colossoma Macropomum Tambaqui
NAL Thesaurus:	aquaculture molecular biology
DOI:	http://dx.doi.org/10.4238/gmr15049228
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAA)