Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1059337
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dc.contributor.authorSILVA, G. A. dapor
dc.contributor.authorBERNARDI, T. L.por
dc.contributor.authorSCHAKER, P. D. C.por
dc.contributor.authorAGUSTINI, B. C.por
dc.contributor.authorMELLO, L. M. R. depor
dc.contributor.authorVALENTE, P.por
dc.contributor.otherGILDO ALMEIDA DA SILVA, CNPUV; Taís Letícia Bernardi, ²Instituto Federal de Educação Ciência e Tecnologia do Rio Grande do Sul, Sertão, Rio Grande do Sul, Brasil; Patrícia Dayane Carvalho Schaker, mbrapa Uva e Vinho - Laboratório de Microbiologia, Bento Gonçalves, Brazil;; BRUNA CARLA AGUSTINI, CNPUV; LOIVA MARIA RIBEIRO DE MELLO, CNPUV; Patrícia Valente, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil.por
dc.date.accessioned2016-12-27T11:11:11Zpor
dc.date.available2016-12-27T11:11:11Zpor
dc.date.created2016-12-27por
dc.date.issued2016por
dc.identifier.other16916por
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1059337por
dc.descriptionThe indiscriminate use of pesticides on grape crops is harmful for consumers´ healthin “in natura” consumption and in the ingestion of wine and grape juice. During winemaking, a rapid and efficient fermentation stage is critical to avoid proliferation of contaminating microorganisms and to guarantee the product´s quality. Polymerase chain reaction (PCR) has the advantage of detecting these contaminants in the early stages of fermentation. However,this enzymatic reaction may also be susceptible to specific problems, reducing its efficiency. Agricultural practices, such as fungicide treatments, may be a source of PCR inhibiting factors and may also interfere in the normal course of fermentation.The action of the pesticides captan and folpet on PCR and on yeast metabolism was evaluated, once these phthalimide compounds are widely employed in Brazilian vineyards. DNA amplification was only observed at 75 and 37.5 µg/mL of captan concentrations, whereas with folpet, amplification was observed only in the two lowest concentrations tested (42.2 and 21.1µg/mL).Besides the strong inhibition on Taq polymerase activity, phthalimides also inhibited yeast metabolism at all concentrations analyzed.Grape must containing captan and folpet residues could not be transformed into wine due to stuck fermentation caused by the inhibition of yeast metabolism. Noncompliance with the waiting period for phthalimide fungicides may result in financial liabilities to the viticulture sector.The use of yeasts with high fungicide sensitivity should be selected for must fermentation as a strategy for sustainable wine production and to assure that products comply with health and food safety standards. Key words: fungicide;PCR inhibitors; stuck fermentation;Taq polymerase inhibitors; yeast growtheng
dc.description.uribitstream/item/152340/1/Gildo-1678-4324-babt-1678-4324-2016160241.pdfpor
dc.languageenpor
dc.language.isoengeng
dc.publisherBrazilian Archives of Biology and Technology, v. 59, e16160241, Jan./Dec. 2016.por
dc.relation.ispartofEmbrapa Uva e Vinho - Artigo em periódico indexado (ALICE)por
dc.rightsopenAccesspor
dc.subjectTaq polymerase inhibitors.por
dc.subjectFungicidepor
dc.subjectPCR inhibitorspor
dc.subjectStuck fermentationpor
dc.titleImpact of fungicide residues on polymerase chain reaction and on yeast metabolism.por
dc.typeArtigo em periódico indexado (ALICE)por
dc.date.updated2019-05-06T11:11:11Zpor
dc.ainfo.id1059337por
dc.ainfo.lastupdate2019-05-06 -03:00:00por
Appears in Collections:Artigo em periódico indexado (CNPUV)

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