Title:	New insights from molecular characterization of the tick Rhipicephalus (Boophilus) microplus in Brazil.
Authors:	CSORDAS, B. G. GARCIA, M. V. CUNHA, R. C. GIACHETTO, P. F. BLECHA, I. M. Z. ANDREOTTI, R.
Affiliation:	BÁRBARA GUIMARÃES CSORDAS, UFMS; MARCOS VALÉRIO GARCIA, Bolsista CNPGC; RODRIGO CASQUERO CUNHA, UFPEL; POLIANA FERNANDA GIACHETTO, CNPTIA; ISABELLA MAIUMI ZAIDAN BLECHA, UFMS; RENATO ANDREOTTI E SILVA, CNPGC.
Date Issued:	2016
Citation:	Revista Brasileira de Parasitologia Veterinaria , Jaboticabal, v. 25, n. 3, p. 317-326, jul./set. 2016.
Description:	The Rhipicephalus (Boophilus) microplus complex currently consists of five taxa, namely R. australis, R. annulatus, R. (B.) microplus clade A sensu, R. microplus clade B sensu, and R. (B.) microplus clade C sensu. Mitochondrial DNA-based methods help Taxonomists when they are facing the morpho-taxonomic problem of distinguishing members of the R. (B.) microplus complex. The purpose of this study was to perform molecular characterization of ticks in all five regions of Brazil and infer their phylogenetic relationships. Molecular analysis characterized 10 haplotypes of the COX-1 gene. Molecular network analysis revealed that haplotype H-2 was the most dispersed of the studied populations (n = 11). Haplotype H-3 (n = 2) had the greatest genetic differentiation when compared to other Brazilian populations. A Bayesian phylogenetic tree of the COX-1 gene obtained strong support. In addition, it was observed that the population of R. (B.) microplushaplotype H-3 exhibited diverging branches among the other Brazilian populations in the study. The study concludes that the different regions of Brazil have R. (B.) microplus tick populations with distinct haplotypes.
NAL Thesaurus:	Rhipicephalus microplus Ticks Brazil
Keywords:	COX-I gene ITS-2 gene Carrapato-do-boi
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGC)