Title:	The use of auxin quantification for understanding clonal tree propagation.
Authors:	STUEPP, C. A. WENDLING, I. TRUEMAN, S. J. KOEHLER, H. S. ZUFFELLATO-RIBAS, K. C.
Affiliation:	CARLOS A. STUEPP, UNIVERSIDADE FEDERAL DO PARANÁ; IVAR WENDLING, CNPF; STEPHEN J. TRUEMAN, CENTRE FOR GENETICS, ECOLOGY AND PHYSIOLOGY, UNIVERSITY OF THE SUNSHINE COAST; HENRIQUE S. KOEHLER, UNIVERSIDADE FEDERAL DO PARANÁ; KATIA C. ZUFFELLATO-RIBAS, UNIVERSIDADE FEDERAL DO PARANÁ.
Date Issued:	2017
Citation:	Forests, v. 5, n. 7, p. 1737-1752, 2017.
Description:	Qualitative and quantitative hormone analyses have been essential for understanding the metabolic, physiological, and morphological processes that are influenced by plant hormones. Auxins are key hormones in the control of many aspects of plant growth and development and their endogenous levels are considered critical in the process of adventitious root induction. Exogenous auxins are used extensively in the clonal propagation of tree species by cuttings or tissue culture. Understanding of auxin effects has advanced with the development of increasingly accurate methods for auxin quantification. However, auxin analysis has been challenging because auxins typically occur at low concentrations, while compounds that interfere with their detection often occur at high concentrations, in plant tissues. Interference from other compounds has been addressed by extensive purification of plant extracts prior to auxin analysis, although this means that quantification methods have been limited by their expense. This review explores the extraction, purification, and quantification of auxins and the application of these techniques in developing improved methods for the clonal propagation of forestry trees.
Keywords:	Adventitious rooting Auxin Cloning Cuttings Indole-3-acetic acid Indolyl-3-acetic acid
DOI:	10.3390/f8010027
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPF)