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dc.contributor.authorDELLAGNEZZE, B. M.pt_BR
dc.contributor.authorVASCONCELLOS, S. P. dept_BR
dc.contributor.authorMELO, I. S. dept_BR
dc.contributor.authorSANTOS NETO, E. V. dospt_BR
dc.contributor.authorOLIVEIRA, V. M. dept_BR
dc.date.accessioned2017-01-30T11:11:11Zpt_BR
dc.date.available2017-01-30T11:11:11Zpt_BR
dc.date.created2017-01-30pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationBrazilian Journal of Microbiology, São Paulo, v. 47, p. 314-321, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1062180pt_BR
dc.descriptionAbstract: Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environmentswith low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices(arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out using Scanning Electronic Microscopy (SEM), Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA gene libraries in order to compare the microbial biomass yield, DNA recovery efficiency and diversity among the enrichments. The DNA from microbial communities in petroleum enrichments was purified according to a protocol established in this work and used for 16S rRNA amplification with bacterial generic primers. The PCR products were cloned, and positive clones were screened by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Sequencing and phylogenetic analyses revealed that the bacterial community was mostly represented by members of the genera Petrotoga, Bacillus, Pseudomonas, Geobacillus and Rahnella. The use of different support materials in the enrichments yielded an increase in microbial biomass and biofilm formation, indicating that these materials may be employed for efficient biomass recovery from petroleum reservoir samples. Nonetheless, the most diverse microbiota were recovered from the biodegraded petroleum sample using polyurethane foam cubes as support material.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBacterial diversitypt_BR
dc.subjectMicrobial enrichmentpt_BR
dc.subjectPetroleum reservoirpt_BR
dc.subjectPhysical supportpt_BR
dc.subject16S rRNApt_BR
dc.titleEvaluation of bacterial diversity recovered from petroleum samples using different physical matrices.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-01-30T11:11:11Zpt_BR
dc.subject.thesagroBactériapt_BR
dc.subject.thesagroBiodiversidadept_BR
dc.subject.thesagroPetróleopt_BR
dc.subject.thesagroBiodegradaçãopt_BR
dc.subject.nalthesaurusBiodiversitypt_BR
riaa.ainfo.id1062180pt_BR
riaa.ainfo.lastupdate2017-01-30pt_BR
dc.identifier.doihttp://dx.doi.org/10.1016/j.bjm.2016.04.004pt_BR
dc.contributor.institutionBRUNA MARTINS DELLAGNEZZE, CPQBA; SUSAN PANTAROTO DE VASCONCELLOS, UNIFESP; ITAMAR SOARES DE MELO, CNPMA; EUGENIO VAZ DOS SANTOS NETO, Petrobras; VALERIA MAIA DE OLIVEIRA, CPQBA.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPMA)

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