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dc.contributor.authorGUIMARÃES-CESTARO, L.pt_BR
dc.contributor.authorSERRÃO, J. E.pt_BR
dc.contributor.authorMESSAGE, D.pt_BR
dc.contributor.authorMARTINS, M. F.pt_BR
dc.contributor.authorTEIXEIRA, É. W.pt_BR
dc.date.accessioned2017-02-08T23:11:19Z-
dc.date.available2017-02-08T23:11:19Z-
dc.date.created2017-02-07pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationJournal of Hymenoptera Research, n. 49, p. 43-50, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1062982pt_BR
dc.descriptionAbstract Honey bees are responsible for pollinating many native and cultivated plant species. These insects can be affected by many pathogens, including fungi and bacteria, both of which can form spores that are easily dispersed within the colony by means of the stored products, among other routes. The objective of this study was to develop a method to detect spores of the honey bee pathogens Nosema apis, Nosema ceranae, Ascosphaera apis and Paenibacillus larvae in samples of honey, bee pollen and royal jelly. The method was standardized for each product individually, and then analyzed by monoplex and multiplex PCR, which showed the same detection thresholds: 1.25 spores/mL of honey for N. ceranae; 7.5 spores/mL of honey for A. apis; and 0.4 spore/mL of honey for P. larvae, respectively. The standardized technique was effective and rapid for the detection of these pathogens in bee products and can be used for the establishment of official methods of sanitary control of bee products, considering the growing national and international trade of these products and the movement or migration of colonies between regions.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBee pathogenspt_BR
dc.subjectDetection of sporespt_BR
dc.titleSimultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-02-08T23:11:19Zpt_BR
dc.subject.thesagroApis Melliferapt_BR
dc.subject.nalthesaurushoneypt_BR
dc.subject.nalthesauruspollenpt_BR
dc.subject.nalthesaurusroyal jellypt_BR
riaa.ainfo.id1062982pt_BR
riaa.ainfo.lastupdate2017-02-07pt_BR
dc.contributor.institutionLubiane Guimarães-Cestaro, UFV; José Eduardo Serrão, UFV; Dejair Message, UFERSA; MARTA FONSECA MARTINS, CNPGL; Érica Weinstein Teixeira, APTA-SAA.pt_BR
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