Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1064560
Research center of Embrapa/Collection: Embrapa Gado de Leite - Artigo em periódico indexado (ALICE)
Date Issued: 2016
Type of Material: Artigo em periódico indexado (ALICE)
Authors: MUNK, M.
LADEIRA, L. O.
CARVALHO, B. C. de
CAMARGO, L. S. de A.
RAPOSO, N. R. B.
SERAPIÁO, R. V.
QUINTAO, C. C. R.
SILVA, S. R.
SOARES, J. S.
JORIO, A.
BRANDAO, H. de M.
Additional Information: Michele Munk, UFJF; Luiz O. Ladeira, UFMG; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Nádia R. B. Raposo, UFJF; Raquel V. Serapião, PESAGRO; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; Saulo R. Silva; Jaqueline S. Soares, UFOP; Ado Jorio, UFMG; HUMBERTO DE MELLO BRANDAO, CNPGL.
Title: Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes.
Publisher: Nature, Scientific Reports 6, Article number: 33588, 2016.
Pages: 10 p.
Language: en
Keywords: Gene delivery
Pellucid zone.
Description: The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.
NAL Thesaurus: in vitro fertilization.
Data Created: 2017-02-16
Appears in Collections:Artigo em periódico indexado (CNPGL)

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