Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1064660
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dc.contributor.authorGIRARDINI, L. K.pt_BR
dc.contributor.authorPAIM, D. S.pt_BR
dc.contributor.authorAUSANI, THAIS C.pt_BR
dc.contributor.authorLOPES, G. V.pt_BR
dc.contributor.authorPELLEGRINI, D. C. P.pt_BR
dc.contributor.authorBRITO, M. A. V. P. ept_BR
dc.contributor.authorCARDOSO, M.pt_BR
dc.date.accessioned2017-02-17T11:11:11Zpt_BR
dc.date.available2017-02-17T11:11:11Zpt_BR
dc.date.created2017-02-17pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationPesquisa Veterinária Brasileira, v. 36, n. 10, p. 951-956, 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1064660pt_BR
dc.descriptionIn intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPFGE clusterspt_BR
dc.subjectMRSApt_BR
dc.titleAntimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-02-20T11:11:11Zpt_BR
dc.subject.nalthesaurusmastitispt_BR
dc.subject.nalthesaurusmethicillin-resistant Staphylococcus aureuspt_BR
riaa.ainfo.id1064660pt_BR
riaa.ainfo.lastupdate2017-02-20pt_BR
dc.contributor.institutionLilian K. Girardini, UNIV. OESTE DANTA CATARINA; Daniel S. Paim, UFRGS; Thais C. Ausani, UFRGS; Graciela V. Lopes, UFRGS; Debora C.P. Pellegrini, UNIV. FEDERAL DO PAMPA; MARIA APARECIDA V PAIVA E BRITO, CNPGL; Marisa Cardoso, UFRGS.pt_BR
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