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dc.contributor.authorFREITAS, R. T. dapt_BR
dc.contributor.authorPAIVA, R.pt_BR
dc.contributor.authorSALES, T. S.pt_BR
dc.contributor.authorSILVA, D. P. C. dapt_BR
dc.contributor.authorREIS, M. V. dospt_BR
dc.contributor.authorSOUZA, A. C. dept_BR
dc.contributor.authorROSA, S. D. V. F. dapt_BR
dc.date.accessioned2017-02-22T11:11:11Zpt_BR
dc.date.available2017-02-22T11:11:11Zpt_BR
dc.date.created2017-02-22pt_BR
dc.date.issued2016pt_BR
dc.identifier.citationNotulac Botanicae Horti Agrobotanici Cluj-Napoca, v. 44, n. 2, p. 445-451, Dec. 2016.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1065188pt_BR
dc.descriptionAs a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectLiquid nitrogenpt_BR
dc.subjectPVS2pt_BR
dc.subjectTTC testpt_BR
dc.titleCryopreservation of Coffea arabica L. zygotic embryos by vitrification.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-03-03T11:11:11Zpt_BR
dc.subject.nalthesaurusEx situ conservationpt_BR
dc.subject.nalthesaurusHistologypt_BR
dc.subject.nalthesaurusThawingpt_BR
riaa.ainfo.id1065188pt_BR
riaa.ainfo.lastupdate2017-03-03pt_BR
dc.contributor.institutionRODRIGO THEREZAN DE FREITAS, UFLA; RENATO PAIVA, UFLA; THAIS SILVA SALES, UFLA; DIOGO PEDROSA CORRÊA DA SILVA, UFLA; MICHELE VALQUÍRIA DOS REIS, UFLA; ANA CRISTINA DE SOUZA, UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC.pt_BR
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