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dc.contributor.authorSOUSA, T. V.pt_BR
dc.contributor.authorCAIXETA, E. T.pt_BR
dc.contributor.authorALKIMIM, E. R.pt_BR
dc.contributor.authorOLIVEIRA, A. C. B. dept_BR
dc.contributor.authorPEREIRA, A. A.pt_BR
dc.contributor.authorZAMBOLIM, L.pt_BR
dc.contributor.authorSAKIYAMA, N. S.pt_BR
dc.date.accessioned2017-03-16T11:11:11Zpt_BR
dc.date.available2017-03-16T11:11:11Zpt_BR
dc.date.created2017-03-16pt_BR
dc.date.issued2017pt_BR
dc.identifier.citationEuphytica, v. 213, n. 3, March, 2017.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1067171pt_BR
dc.descriptionNew cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes sing morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness of markers in distinguishing related genotypes from those with similar phenotypic traits. This biotechnological tool will assist breeders in DUS testing of cultivars.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMolecular profilingpt_BR
dc.subjectProtection of plant cultivarspt_BR
dc.subjectCultivars registerpt_BR
dc.subjectSSR markerpt_BR
dc.titleMolecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-12-15T11:11:11Zpt_BR
dc.subject.nalthesaurusCoffeapt_BR
dc.subject.nalthesaurusDNA fingerprintingpt_BR
dc.subject.nalthesaurusGenetic variationpt_BR
dc.subject.nalthesaurusCultivar identificationpt_BR
riaa.ainfo.id1067171pt_BR
riaa.ainfo.lastupdate2017-12-15 -02:00:00pt_BR
dc.contributor.institutionTIAGO VIEIRA SOUSA, BIOAGRO, Biocafé/UFV; EVELINE TEIXEIRA CAIXETA, SAPC; EMILLY RUAS ALKIMIM, BIOAGRO, Biocafé/UFV; ANTONIO CARLOS BAIAO DE OLIVEIRA, SAPC; ANTONIO ALVES PEREIRA, EPAMIG; LAÉRCIO ZAMBOLIM, UFV; NEY SUSSUMU SAKIYAMA, UFV.pt_BR
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