Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1071596
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dc.contributor.authorSILVA, J. M. Fpt_BR
dc.contributor.authorRWAHNIH, M. A.pt_BR
dc.contributor.authorBLAWID, R.pt_BR
dc.contributor.authorNAGATA, T.pt_BR
dc.contributor.authorFAJARDO, T. V. M.pt_BR
dc.date.accessioned2017-06-27T11:11:11Zpt_BR
dc.date.available2017-06-27T11:11:11Zpt_BR
dc.date.created2017-06-27pt_BR
dc.date.issued2017pt_BR
dc.identifier.citationVirus Genes, v.53, n. 4, p. 667-671 [online], 2017.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1071596pt_BR
dc.descriptionIn this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remark-able identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3 0 untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus . Keywords High-throughput sequencing Virus discovery Luteoviridae Grapevine enamovirus-1 GEV-1pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectHigh-throughput sequencingpt_BR
dc.subjectGrapevine enamovirus-1pt_BR
dc.subjectGEV-1pt_BR
dc.titleDiscovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2019-05-06T11:11:11Zpt_BR
dc.subject.nalthesaurusLuteoviridaept_BR
riaa.ainfo.id1071596pt_BR
riaa.ainfo.lastupdate2019-05-06 -03:00:00pt_BR
dc.identifier.doi10.1007/s11262-017-1470-ypt_BR
dc.contributor.institutionJoão Marcos Fagundes Silva, University of Bras?lia, Bras?lia, DF, Brazil; Maher Al Rwahnih, Department of Plant Pathology, University of California, Davis, CA, USA; Rosana Blawid, University of Bras? ? lia, Bras? ? lia, DF, Brazil; Tatsuya Nagata, University of Bras?lia, Bras?lia, DF, Brazil; THOR VINICIUS MARTINS FAJARDO, CNPUV.pt_BR
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