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dc.contributor.authorSOUZA, E. B.pt_BR
dc.contributor.authorNICKEL, O.pt_BR
dc.contributor.authorFAJARDO, T. V. M.pt_BR
dc.contributor.authorSILVA, J. M. F.pt_BR
dc.contributor.authorBARROS, D. R.pt_BR
dc.date.accessioned2017-06-28T11:11:11Zpt_BR
dc.date.available2017-06-28T11:11:11Zpt_BR
dc.date.created2017-06-28pt_BR
dc.date.issued2017pt_BR
dc.identifier.citationTropical Plant Pathology, v. 42, n. 5, p. 391-396, 2017.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1071609pt_BR
dc.descriptionApple stem grooving virus (ASGV, genus Capillovirus) is disseminated worldwide, usually causing a latent infection in most commercial apple cultivars. However, infected scions grafted onto sensitive material display reduction of yield, loss of fruit quality and tree decline. In Brazil ASGV is associated with severe phloem necrosis, xylem pitting and decline of apple trees on Maruba-kaido (Malus prunifolia cv. Ringo) rootstocks usually in a complex with other latent viruses. Two Brazilian ASGV isolates from a mixed infection causing differing reactions on apple cv. Fuji on Maruba-kaido rootstocks, i.e., normal growth vs. tree decline, have been completely sequenced. The differing intensity of symptoms they co-induced on several woody indicator species/cultivars are indicative of biological differences between the two inocula. Their nucleotide sequences are 92.2% identical and display between 79.2% and 97.1% identity with other ASGV isolates available in GenBank. The two isolates (M219-3 and M220) shared 92.3% deduced amino acid (daa) sequence identity for ORF1, 95.3% for ORF2 and 93.3% for the CP gene. A high degree of daa identity, 95.8% and 96.6%, was ob- served between the CPs of M219 ? 3 and M220, respectively, with the Brazilian isolate UV01. Potential recombination events and phy logenetic relationships to major parents were investigated in isolates M219 ? 3and M220. Keywords ASGV . Apple virus . Phylogenetic analysis . Recombinationpt_BR
dc.language.isoengpt_BR
dc.rightsopenAccesseng
dc.subjectApple viruspt_BR
dc.subjectASGV (Apple stem grooving virus)pt_BR
dc.subjectPhylogenetic analysispt_BR
dc.subjectRecombinationpt_BR
dc.titleBiological and molecular characterization of two Brazilian isolates of Apple stem grooving virus.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2019-05-06T11:11:11Zpt_BR
riaa.ainfo.id1071609pt_BR
riaa.ainfo.lastupdate2019-05-06 -03:00:00pt_BR
dc.identifier.doi10.1007/s40858-017-0163-3pt_BR
dc.contributor.institutionElen B. Souza, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF 70910-900, Brazil; OSMAR NICKEL, CNPUV; THOR VINICIUS MARTINS FAJARDO, CNPUV; João M. F. Silva, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF 70910-900, Brazil; Danielle R. Barros, Departamento de Fitossanidade, Universidade Federal de Pelotas, Pelotas, RS 96010-900, Brazil.pt_BR
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