Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1071896
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dc.contributor.authorFANÉ, R.pt_BR
dc.contributor.authorKASSOGUÉ, A.pt_BR
dc.contributor.authorTRAORÉ, D.pt_BR
dc.contributor.authorDAO, S.pt_BR
dc.contributor.authorMAÏGA, K.pt_BR
dc.contributor.authorBAH, A.pt_BR
dc.contributor.authorVALICENTE, F. H.pt_BR
dc.contributor.authorBARBANA, A. H.pt_BR
dc.date.accessioned2017-07-03T11:11:11Zpt_BR
dc.date.available2017-07-03T11:11:11Zpt_BR
dc.date.created2017-07-03pt_BR
dc.date.issued2017pt_BR
dc.identifier.citationAfrican Journal of Biotechnology, v. 16, n. 17, p. 983-988, 2017.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1071896pt_BR
dc.descriptionThe objective of this work was to select the most active Bacillus thuringiensis (Bt) isolated from agricultural soils of Mali through the molecular characterization and the determination of insecticidal activities of the protein crystals, produced by these native isolates. Crystal proteins were extracted from B. thuringiensis culture, and characterized using the SDS-PAGE techniques. Their insecticidal activities were tested using third-instar larvae of Helicoverpa armigera in bioassay tests. The results showed that, of 62 B. thuringiensis treated, 52 isolates showed fragments varying between 10 and 140 kDa on 12% polyacrylamide gel. Cry1 and Cry2 protein crystals were recognized to be effective against Lepidoptera?s larvae, which were found in 21% of the tested isolates. In addition to these two expected crystal protein weights, other molecular weights were observed at different proportions, suggesting the presence of other cry genes in the local B. thuringiensis isolates. Four native B. thuringiensis isolates were able to kill 95 to 100% of H. armigera 3rd-instar larvae. Only one native of B. thuringiensis isolate was able to kill 100% of the H. armigera larvae. This is the first study for molecular characterization of Malian native B. thuringiensis isolates, showing the efficacy of the native B. thuringiensis against an important agricultural insect pest.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleMolecular characterization and insecticidal activities of Malian native crystalliferous Bacillus thuringiensis.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-07-03T11:11:11Zpt_BR
dc.subject.thesagroBacteriapt_BR
dc.subject.thesagroControle biológicopt_BR
riaa.ainfo.id1071896pt_BR
riaa.ainfo.lastupdate2017-07-03pt_BR
dc.identifier.doi10.5897/AJB2017.15908pt_BR
dc.contributor.institutionFERNANDO HERCOS VALICENTE, CNPMS.pt_BR
Appears in Collections:Artigo em periódico indexado (CNPMS)

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